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首页> 外文期刊>The British Journal of Nutrition >Identification of intra- and inter-individual metabolite variation in plasma metabolite profiles of cats and dogs.
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Identification of intra- and inter-individual metabolite variation in plasma metabolite profiles of cats and dogs.

机译：鉴定猫和狗血浆代谢物谱中个体间和个体间的代谢物变化。

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The purpose of the present study was first to identify drivers of variance in plasma metabolite profiles of cats and dogs that may affect the interpretation of nutritional metabolomic studies. A total of fourteen cats and fourteen dogs housed in environmentally enriched accommodation were fed a single batch of diet to maintain body weight. Fasting blood samples were taken on days 14, 16 and 18 of the study. Gas chromatography-mass spectrometry (GC-MS), liquid chromatography (LC)-MS/MS and solid-phase extraction-LC-MS/MS analyses were used for metabolite profiling. Principal component (PC) analysis that indicated 31 and 27% of the variance was explained in PC1 and PC2 for cats and dogs, respectively, with most individuals occupying a unique space. As the individual was a major driver of variance in the plasma metabolome, the second objective was to identify metabolites associated with the individual variation observed. The proportion of intra- and inter-individual variance was calculated for 109 cat and 101 dog metabolites with a low intra-individual variance (SD<0.05). Of these, fifteen cat and six dog metabolites had inter-individual variance accounting for at least 90% of the total variance. There were four metabolites common to both species (campesterol, DHA, a cholestenol and a sphingosine moiety). Many of the metabolites with >75% inter-individual variance were common to both species and to similar areas of metabolism. In summary, the individual is an important driver of variance in the fasted plasma metabolome, and specific metabolites and areas of metabolism may be differentially regulated by individuals in two companion animal species.
机译：本研究的目的是首先确定猫和狗血浆代谢物谱中可能影响营养代谢组学研究解释的差异驱动因素。为总共十四只猫和十四只狗提供了丰富的饮食，以维持体重。在研究的第14、16和18天采集空腹血液样品。气相色谱-质谱（GC-MS），液相色谱（LC）-MS / MS和固相萃取-LC-MS / MS分析用于代谢物分析。分别在猫和狗的PC1和PC2中解释了表明分别有31％和27％的方差的主成分（PC）分析，大多数人都占据了一个独特的空间。由于个体是血浆代谢组变异的主要驱动因素，因此第二个目标是鉴定与观察到的个体变异相关的代谢产物。计算个体内差异低的SD猫和101只狗代谢物的个体内差异和个体间差异的比例（SD <0.05）。其中，15种猫和6种狗代谢物的个体间差异至少占总差异的90％。两种物种共有四种代谢物（菜油甾醇，DHA，胆固醇和鞘氨醇部分）。个体间差异大于75％的许多代谢物对于两个物种和相似的代谢区域都是相同的。总之，个体是禁食血浆代谢组中差异的重要驱动因素，特定代谢物和代谢区域可能受到两种同伴动物物种中个体的差异调节。

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