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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Separation of Flavonoids by Semi-Micro High-Performance Liquid Chromatography with Electrochemical Detection
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Separation of Flavonoids by Semi-Micro High-Performance Liquid Chromatography with Electrochemical Detection

机译：半微量高效液相色谱-电化学检测法分离类黄酮

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摘要

A highly sensitive and selective method was developed for the determination of 15 flavonoids by high-performance liquid chromatography with electrochemical detection(HPLC-ECD)using a microbore column.The 15 flavonoids were divided into two groups according to their hydrophobicity and were resolved by two isocratic systems,namely,methanol-water(1:1 and 3:7,v/v)containing 0.5% phosphoric acid.The retention factor(k)of each flavonoid linearly correlated with the logarithmic partition of coefficients between 1-octanol-water(logp)values.The detection limits(S/N=3)of the flavonoids tested were in the range of 2-25 fmol;that is 600 times more sensitive than conventional HPLC with UV detection.The relative standard deviations(RSD,n=5)were less than 5% at 1 pmol for the flavonoids tested.By the present method,only 100 muL of bottled Japanese green tea were enough to determine the flavonoid contents.
机译：建立了一种高效灵敏，选择性强的高效液相色谱-微孔色谱-电化学检测（HPLC-ECD）法测定15种黄酮的方法，将15种黄酮按疏水性分为两组，分别用两种方法拆分等度体系，即含有0.5％磷酸的甲醇-水（1：1和3：7，v / v）。每种类黄酮的保留因子（k）与1-辛醇-水之间系数的对数分配线性相关（logp）值。测试的类黄酮的检出限（S / N = 3）在2-25 fmol范围内;这是使用紫外线检测的常规HPLC灵敏度的600倍。相对标准偏差（RSD，n = 5）的类黄酮含量在1 pmol时小于5％。通过本方法，仅100μL瓶装日本绿茶足以确定类黄酮含量。

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