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首页> 外文期刊>Biomaterials Science >Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids
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Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

机译：多层肝素涂层的生物分子呈递给人类间充质干细胞球体的表征

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摘要

Mesenchymal stem cell (MSC) therapies have the potential to treat many pathologies, however, controlling cell fate after implantation remains challenging. We have used a multilayer technology to graft a range of 5 μg mL~(-1)-5 mg mL~(-1) heparin onto the surface of MSC aggregates. Heparin coating does not affect cell viability (seen through LIVE/DEAD staining) and cell anti-inflammatory properties (seen through co-culture with activated monocytes) and facilitates sequestration by coated cells of a growth factor (TGF-β1) that remains bioactive. This system can maximize the therapeutic potential of MSC-based treatments because the cell surface-loaded protein could both signal the cells to influence transplanted cell fate and be released into the surrounding environment to help repair injured tissue.
机译：间充质干细胞（MSC）疗法具有治疗多种病理的潜力，但是，植入后控制细胞命运仍然具有挑战性。我们已使用多层技术将5μgmL〜（-1）-5 mg mL〜（-1）肝素移植到MSC聚集体表面上。肝素涂层不会影响细胞活力（通过LIVE / DEAD染色可见）和细胞抗炎特性（通过与活化的单核细胞共培养可见），并有助于被包被细胞隔离具有生物活性的生长因子（TGF-β1）。该系统可以最大程度地提高基于MSC的治疗方法的治疗潜力，因为细胞表面加载的蛋白质既可以发信号通知细胞影响移植的细胞命运，又可以释放到周围环境中以帮助修复受损的组织。

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