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首页> 外文期刊>Biomaterials Science >Hydrogelation of dextran-based polyampholytes with cryoprotective properties via click chemistry
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Hydrogelation of dextran-based polyampholytes with cryoprotective properties via click chemistry

机译：通过点击化学具有防冻性能的基于葡聚糖的两性电解质水凝胶化

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摘要

Hydrogels are promising substrates for tissue engineering applications because of their unique biocompatibility, flexible methods of synthesis, range of constituents, and desirable physical characteristics. Cryopreservation of cell-containing constructs using such hydrogel scaffolds is in high demand in tissue-engineering applications for the production of "off-the-shelf" tissue-engineered products. However, cryopreservation of regenerated tissues including cell sheets and cell constructs is not easy compared to the preservation of cell suspensions, even when cryoprotectants are used. Here, we report a dextranbased polyampholyte hydrogel that itself shows cryoprotective properties, which could be useful for cell encapsulation and tissue engineering applications involving hydrogel formation. Amination was performed by introducing poly-L-lysine onto azide groups conjugated with dextran, and a portion of the amino groups was converted into carboxyl groups. These dextran-based polyampholytes showed good cryoprotective properties for mammalian cells, and the addition of dextran substituted with dibenzylcyclooctyne acid induced in situ hydrogel formation via Cu-free click chemistry with high biocompatibility. Cells encapsulated with such in situ hydrogels can be cryopreserved well without the addition of any cryoprotectants. Thus, these hydrogels can serve as scaffolds with cryoprotective properties that also provide structural integrity to tissue constructs.
机译：水凝胶因其独特的生物相容性，灵活的合成方法，组成范围和理想的物理特性而成为有希望用于组织工程应用的基质。在组织工程应用中，对于生产“现成的”组织工程产品，对使用这种水凝胶支架进行含细胞构建体的冷冻保存有很高的要求。然而，即使使用冷冻保护剂，与细胞悬浮液的保存相比，包括细胞片和细胞构建体在内的再生组织的冷冻保存也不容易。在这里，我们报道了一种基于葡聚糖的聚两性电解质水凝胶，它本身具有防冻性能，可用于涉及水凝胶形成的细胞封装和组织工程应用。通过将聚-L-赖氨酸引入与葡聚糖缀合的叠氮化物基团上来进行胺化，并且一部分氨基被转化为羧基。这些基于葡聚糖的多两性电解质对哺乳动物细胞显示出良好的冷冻保护性能，并且通过用无铜点击化学法以高生物相容性加入被二苄基环辛炔酸取代的葡聚糖诱导原位水凝胶形成。用这种原位水凝胶封装的细胞可以很好地冷冻保存，而无需添加任何冷冻保护剂。因此，这些水凝胶可以用作具有冷冻保护特性的支架，该支架还为组织构建体提供结构完整性。

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