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首页> 外文期刊>Biomaterials Science >Modulation of neuronal network activity using magnetic nanoparticle-based astrocytic network integration
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Modulation of neuronal network activity using magnetic nanoparticle-based astrocytic network integration

机译：使用基于磁性纳米粒子的星形细胞网络集成调节神经元网络活动

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Investigating the mechanisms of the neuron-glia interaction is important in the basic research of neuroscience and neural transplantation. Synaptic transmission is modulated by astrocyte activation in the pre- and post-synaptic terminals, and this phenomenon is spread to the surrounding astrocytes through gap junctions. However, the modulation of network-wide neuronal activity dependent on extensive astrocyte activation is not well understood. In this study, we show network-wide neuronal modulation associated with a newly developed three-dimensional neuronal and astrocytic network co-culture method. To establish widespread neuronal and astrocytic network interactions in vitro, we performed integration of magnetic nanoparticle-injected astrocytes (Mag-AS) onto the matured monolayer of neuronal networks using an external magnetic force. The neuronal electrical activity was dynamically synchronized at 24 h after integration of the Mag-AS network. In addition, Mag-AS network activation using a caged calcium compound rapidly induced suppression and subsequent synchronization of neuronal electrical activity. These results indicate that the high-density astrocytic network integration onto the neuronal network can induce widespread neuronal modulation, and our in vitro co-culture method contributes to the advancement of neuronal and astrocytic transplantation research.
机译：在神经科学和神经移植的基础研究中，研究神经元-胶质细胞相互作用的机制很重要。突触传递受到突触前和突触后末端中星形胶质细胞激活的调节，这种现象通过间隙连接扩散到周围的星形胶质细胞。但是，依赖于广泛的星形胶质细胞活化的网络范围神经元活性的调节尚不十分清楚。在这项研究中，我们显示了与新开发的三维神经元和星形细胞网络共培养方法相关的全网络神经元调制。为了在体外建立广泛的神经元和星形细胞网络相互作用，我们使用外部磁力将注射了磁性纳米粒子的星形胶质细胞（Mag-AS）整合到了成熟的神经元网络单层中。在整合Mag-AS网络后的24小时，神经元的电活动被动态同步。此外，使用笼状钙化合物的Mag-AS网络激活会迅速诱导抑制作用，并随后同步神经元电活动。这些结果表明高密度的星形细胞网络整合到神经元网络可以诱导广泛的神经元调节，我们的体外共培养方法有助于神经元和星形细胞移植研究的发展。

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