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首页> 外文期刊>Biomaterials Science >Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film
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Priming cells for their final destination: microenvironment controlled cell culture by a modular ECM-mimicking feeder film

机译：为细胞到达最终目的地而准备：通过模块化ECM模拟饲养膜对微环境进行控制的细胞培养

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摘要

Mammalian cell culture is the starting point in many research studies focusing on biomedical applications. However, researchers have little control over the standardized cell microenvironment parameters. Here a modular ECM-mimicking surface coating for cell culture environment is designed. This substrate is a new and versatile thin film obtained by spin-coating of concentrated gelatin crosslinked by transglutaminase. It can be modified with respect to the biochemical and biophysical needs of the final cell destination, i.e. it delivers loaded multi-growth factors and serum components and allows for cell culture in a serum-free culture medium. Also, a well-known cell behavior modulator, the substrate stiffness, is controlled exogenously by addition of nanoparticles. In addition to growth factors, antimicrobial agents such as natural peptides are added to the substrate for limiting the repeated addition of antimicrobial agents to the culture medium and to prevent the increase of resistant bacterial strains in the culture environment. Finally, this substrate contains simultaneously ECM components, growth factors, stiffening elements and antimicrobial agents. It provides a favorable microenvironment and sterile conditions. It is a free-of-maintenance system, as cells will grow without addition of serum or antimicrobial cocktails. This low cost and easy-to-use substrate could emerge as a new standard for cell culture.
机译：哺乳动物细胞培养是许多专注于生物医学应用的研究的起点。但是，研究人员对标准化的细胞微环境参数几乎没有控制权。在这里设计了用于细胞培养环境的模块化ECM模拟表面涂层。该基质是通过旋涂通过转谷氨酰胺酶交联的浓缩明胶获得的新型多功能薄膜。可以根据最终细胞目的地的生化和生物物理需求对其进行修改，即，它可以传递负载的多种生长因子和血清成分，并允许在无血清培养基中进行细胞培养。而且，众所周知的细胞行为调节剂，即底物硬度，是通过添加纳米颗粒来外源控制的。除生长因子外，将抗微生物剂（例如天然肽）添加到基质中，以限制将抗微生物剂重复添加到培养基中，并防止培养环境中耐药细菌菌株的增加。最后，该基材同时包含ECM成分，生长因子，硬化元素和抗菌剂。它提供了有利的微环境和无菌条件。它是免维护系统，因为细胞将在不添加血清或抗微生物混合物的情况下生长。这种低成本且易于使用的底物可能会成为细胞培养的新标准。

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