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首页> 外文期刊>The Indian Journal of Animal Reproduction >EFFECT OF FERTILITY ASSOCIATED PROTEIN ON OXIDATIVE STRESS OF BOVINE SPERM CELLS
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EFFECT OF FERTILITY ASSOCIATED PROTEIN ON OXIDATIVE STRESS OF BOVINE SPERM CELLS

机译：生育相关蛋白对牛精子细胞氧化应激的影响

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摘要

The present study addressed the effects of fertility associated protein on oxidative stress in frozen thawed bull spermatozoa. The frozen semen samples of 22 bulls were thawed at 37° C in a water bath and oxidative stress was measured indirectly by estimating malondialdehyde (MDA) level at immediate post thaw, 60,120 and 180 min of incubation. To find whether addition of fertility associated protein would control the oxidative stress of the sperm cells, 25 ug of 28 - 30 kDa heparin binding protein of the sperm membrane was added to the thawed semen samples and MDA levels were estimated. MDA level was significantly lower (P < 0.01) in fertility associated protein treated semen sample than the untreated control semen at 60 min (1.95 ± 0.11 vs 2.85 ± 0.12), 120 min (2.40 ± 0.12 vs 3.20 ± 0.20) and 180 of incubation (2.99 ± 0.15 vs 4.31 ± 0.32). It was concluded that 28 - 30 kDa heparin binding protein of the sperm membrane could help in controlling the oxidative stress of the sperm cells duringincubation.
机译：本研究解决了生育相关蛋白对冷冻解冻的公牛精子氧化应激的影响。将22头公牛的冷冻精液样品在37°C的水浴中解冻，并通过在解冻后60,120和180分钟立即评估丙二醛（MDA）水平来间接测量氧化应激。为了发现是否添加与生育力相关的蛋白质可以控制精子细胞的氧化应激，将25 ug精子膜的28-30 kDa肝素结合蛋白添加到解冻的精液样本中，并估算MDA水平。与生育相关蛋白处理的精液样品中的MDA水平在60分钟（1.95±0.11 vs 2.85±0.12），120分钟（2.40±0.12 vs 3.20±0.20）和180度温育时显着低于未处理的对照精液（P <0.01） （2.99±0.15与4.31±0.32）。结论是，精子膜上的28-30 kDa肝素结合蛋白可以帮助控制温育过程中精子细胞的氧化应激。

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