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首页> 外文期刊>The Indian Journal of Animal Reproduction >EFFECT OF LEPTIN ON IN-VITRO MATURATION OF OOCYTES AND ON EARLY EMBRYONIC DEVELOPMENT IN BUFFALOES
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EFFECT OF LEPTIN ON IN-VITRO MATURATION OF OOCYTES AND ON EARLY EMBRYONIC DEVELOPMENT IN BUFFALOES

机译：瘦素对水牛卵卵母细胞体外成熟及早期胚发育的影响

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摘要

The present study was aimed to assess the physiological function of Leptin on in-vitro maturation of oocytes and early embryonic development to increase the efficiency of in-vitro embryo production in buffaloes. The oocytes were maturated in 199 medium supplemented with 10% FBS, 0.5ug/ml FSH, 10IU/ml LH, 1 ug/ml estradiol-17p, 20ng/ml EGF and 50 uM cysteamine in four groups i.e. Group-I (without leptin), Group-ll (10ng/ml leptin), Group-Ill (20ng/ml leptin) and Group-IV (30ng/ml leptin) in C02 incubator at 37°C temperature, 5% C02and high humidity. The matured oocytes were fertilized in-vitrow modified synthetic oviductal fluid (mSOF) containing 10ng/ml heparin. Presumptive zygotes were cultured in mSOF for 7 days to study embryonic development. The oocytes maturation rates were 76.55, 83.25, 90.14 and 92.16 %, respectively in groups I, II, III and IV, cleavage rates were 48.75, 59.77, 70.83 and 71.50%, embryonic development to morula stage were 41.03, 50.96, 58.82 and 57.34%% and blastocyst development rates were 25.64,30.77,44.12 and 44.06 %. The oocytes maturation, cleavage, morula and blastocyst development rates were significantly higher in leptin supplemented groups. From the present study, it may be concluded that leptin at the concentration of 20ng/ml in maturation medium significantly (P<0.05) improved maturation rate, cleavage, morula and blastocyst development in buffaloe oocytes.
机译：本研究旨在评估瘦素对卵母细胞体外成熟和早期胚胎发育的生理功能，以提高水牛体外胚胎生产的效率。卵母细胞在四组即I组（不含瘦素）补充10％FBS，0.5ug / ml FSH，10IU / ml LH，1ug / ml雌二醇-17p，20ng / ml EGF和50uM半胱胺的199培养基中成熟。 ），II组（10ng / ml瘦素），III组（20ng / ml瘦素）和IV组（30ng / ml瘦素）在37°C温度，5％CO2和高湿度的CO2培养箱中。将成熟的卵母细胞体外修饰含有10ng / ml肝素的人工合成输卵管液（mSOF）。假定合子在mSOF中培养7天，以研究胚胎发育。 I，II，III和IV组卵母细胞成熟率分别为76.55％，83.25、90.14和92.16％，卵裂率分别为48.75、59.77、70.83和71.50％，胚胎发育到桑ula壳阶段分别为41.03、50.96、58.82和57.34。 %%和胚泡发育率分别为25.64、30.77、44.12和44.06％。在补充瘦素的组中，卵母细胞的成熟，卵裂，桑和胚泡发育速率显着更高。从本研究中可以得出结论，在成熟培养基中浓度为20ng / ml的瘦素可以显着改善（P <0.05）水牛卵母细胞的成熟率，卵裂，桑ula和胚泡发育。

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