首页> 外文期刊>The Indian Journal of Animal Reproduction >EFFECT OF CORPUS LUTEUM AND STATUS OF ANIMAL ON RECOVERY RATE OF OOCYTES IN OVINE
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EFFECT OF CORPUS LUTEUM AND STATUS OF ANIMAL ON RECOVERY RATE OF OOCYTES IN OVINE

机译:黄体和动物状态对卵中卵细胞恢复率的影响

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Genital organs of slaughtered ewes were collected from abattoirs in Srinagar city and transported to the laboratory in warm normal saline solution containing 50mug gentamicin sulphate per ml. Upon arrival to the laboratory; the status of the reproductive tract (gravid or non-gravid) and ovaries for corpus luteum (with or without) were observed and recorded. The oocytes were collected separately from ovaries of gravid and non-gravid uteri as well as from ovaries with or without corpus luteum (CL) using slicing method and graded as good, fair and poor for each ovary under stereo-zoom microscope. The good quality, usable and total oocytes harvested from ovaries without CL were significantly (p <0.05) higher (3.13,4.91 and 6.57) than the ovaries containing CL (2.55,4.14 and 5.62). However, the number of good quality, usable and total oocytes recovered was non-significantly (p >0.05) higher for ovaries of non-gravid uteri (3.14,4.92 and 6.45) than for ovaries of gravid uterus (1.96, 3.38 and 5.04). In conclusion, ovaries without CL were better for oocyte collection from abattoir ovaries in ovine.
机译:从斯利那加市的屠宰场收集了被屠宰的母羊的生殖器官,并在温暖的生理盐水溶液中(每毫升含50μg硫酸庆大霉素)运送到实验室。到达实验室后;观察并记录生殖道(重度或非重度)和黄体卵巢(有或没有)的状态。使用切片方法分别从妊娠子宫和非妊娠子宫的卵巢以及有或没有黄体(CL)的卵巢中收集卵母细胞,并在体视变焦显微镜下将每个卵巢的卵母细胞分为好,好和差。从没有CL的卵巢中收获的高质量,可用和总卵母细胞比含有CL的卵巢(2.55、4.14和5.62)显着高(p <0.05)(3.13、4.91和6.57)。然而,非妊娠子宫的卵巢(3.14、4.92和6.45)的质量,可用卵母细胞和总卵母细胞的回收率显着高于妊娠子宫的卵巢(1.96、3.38和5.04)(p> 0.05) 。总之,没有CL的卵巢更适合从绵羊的屠宰场卵巢中收集卵母细胞。

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