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首页> 外文期刊>The Indian Journal of Animal Reproduction >EFFECT OF DILUTORS ON MOTILITY, MORPHOLOGYAND ACROSOME INTEGRITY OF RAM SPERMATOZOADURING FREEZING
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EFFECT OF DILUTORS ON MOTILITY, MORPHOLOGYAND ACROSOME INTEGRITY OF RAM SPERMATOZOADURING FREEZING

机译：稀释剂对冻结过程中RAM精子的运动性，形态和原子完整性的影响

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摘要

The effects of dilutors, rams, stages of freezing process and their interactions were studied in 90 ejaculates in a 3x5x3 factorial experiment for biophysical characteristics of sperms during freezing of sheep semen. The diluents used was 20% egg yolkand 4% glycerol with tris fructose citric acid. (TFCEG), a synthetic phosphate medium (Phos) and sodium citrate glucose egg yolk glycerol (SCGEG). The influences of dilutors, rams, stages of freezing and interactions of dilutorwith ram, and dilutorwithstages of freezing were highly significant (P<0.01) on motility and abnormal sperm as well as acrosome score. Maximum post-thaw motility (36.67 ± 1.13%) was observed in TFCEG diluent followed by Phos (31.00 ± 1.43%) and the least in SCGEG (19.83 ± 0.85%) diluent. Rams having better sperm motility at dilution stage could express equally good post-thaw motility in Phos diluent. The percentages of morphologically abnormal sperms and acrosome scores were also significantly (P<0.05) influenced by the dilutors, being minimum in Phos and maximum in TFCEG diluent. Further, the observations indicated that TFCEG provided better protection to sperm motility whereas Phos diluent provided maximum protection to acrosme morphology while cryofreezing of ram semen.
机译：在3x5x3析因实验中，在90个精液中研究了稀释剂，公羊，冷冻过程的阶段及其相互作用的影响，以研究绵羊精液冷冻过程中精子的生物物理特性。所用的稀释剂是20％的蛋黄和4％的甘油与三果糖柠檬酸。 （TFCEG），合成磷酸盐培养基（Phos）和柠檬酸钠葡萄糖蛋黄甘油（SCGEG）。稀释剂，公羊，冷冻阶段以及稀释剂与公羊的相互作用以及稀释剂与冷冻阶段的相互作用对活力，精子异常以及顶体得分的影响均具有显着性（P <0.01）。在TFCEG稀释剂中观察到最大的解冻后活力（36.67±1.13％），其次是Phos（31.00±1.43％），而在SCGEG中则最小（19.83±0.85％）。在稀释阶段精子活力更好的公羊在Phos稀释剂中可以表现出同样好的融化后活力。稀释剂对形态异常精子的百分比和顶体得分的影响也显着（P <0.05），其中Phos最低，TFGEG稀释剂最高。此外，观察结果表明，TFMEG对精子活力提供了更好的保护，而Phos稀释剂则在冷冻精子时为顶体形态提供了最大的保护。

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