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首页> 外文期刊>Biomaterials Science >Surfaces presenting alpha-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic Escherichia coli biofilms against pathogen colonization
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Surfaces presenting alpha-phenyl mannoside derivatives enable formation of stable, high coverage, non-pathogenic Escherichia coli biofilms against pathogen colonization

机译：呈现α-苯基甘露糖苷衍生物的表面能够形成稳定，高覆盖度，无病原性的大肠杆菌生物膜，以抵抗病原体的定殖

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Prevention of pathogenic colonization on medical devices over a long period of time remains a great challenge, especially in a high-nutrient environment that accelerates the production of biomass leading to biofouling of the device. Since biofouling and the subsequent pathogen colonization is eventually inevitable, a new strategy using non-pathogenic bacteria as living guards against pathogenic colonization on medical devices has attracted increasing interest. Crucial to the success of this strategy is to pre-establish a high coverage and stable biofilm of benign bacteria on the surface. Silicone elastomers are one of the most widely used materials in biomedical devices. In this work, we modified silicone surfaces to promote formation of high coverage and stable biofilms by a non-pathogenic Escherichia coli strain 83972 with type 1 fimbriae (fim+) to interfere with the colonization of an aggressive biofilm-forming, uropathogenic Enterococcus faecalis. Although it is well known that mannoside surfaces promote the initial adherence of fim+ E. coli through binding to the FimH receptor at the tip of the type 1 fimbriae, it is not clear whether the fast initial adherence could lead to a high coverage and stable protective biofilm. To explore the role of mannoside ligands, we synthesized a series of alkyl and aryl mannosides varied in the structure and immobilized them on silicone surfaces pre-coated with a poly(amidoamine) (PAMAM) dendrimer. We found that stable and densely packed benign E. coli biofilms were formed on the surfaces presenting biphenyl mannoside with the highest initial adherence of fim+ E. coli. These non-pathogenic biofilms prevented the colonization of E. faecalis for 11 days at a high concentration (10(8) CFU mL(-1), 100 000 times above the diagnostic threshold for urinary tract infection) in the nutrient-rich Lysogeny Broth (LB) media. The result shows a correlation among the initial adherence of fim+ E. coli 83972, the coverage and long-term stability of the resulting biofilms, as well as their efficiency for preventing the pathogen colonization.
机译：长期防止在医疗器械上致病性定植仍然是一个巨大的挑战，特别是在高营养环境中，该环境会加速生物质的产生，从而导致器械的生物污染。由于最终将不可避免地发生生物污染和随后的病原体定殖，因此使用非病原性细菌作为在医疗器械上防止病原体定殖的有效措施的新策略引起了越来越多的兴趣。该策略成功的关键是在表面上预先建立高覆盖度和稳定的良性细菌生物膜。硅酮弹性体是生物医学设备中使用最广泛的材料之一。在这项工作中，我们修改了有机硅表面，以促进非致病性大肠杆菌83972与1型菌毛（fim +）的高覆盖度和稳定的生物膜形成，以干扰具有攻击性的生物膜形成，尿路致病性肠球菌的定植。尽管众所周知，甘露糖苷表面通过与1型菌毛尖端的FimH受体结合而促进fim +大肠杆菌的初始粘附，但尚不清楚快速的初始粘附是否会导致高覆盖率和稳定的保护作用生物膜。为了探索甘露糖苷配体的作用，我们合成了一系列结构不同的烷基和芳基甘露糖苷，并将其固定在预先涂有聚（酰胺基胺）（PAMAM）树枝状大分子的有机硅表面上。我们发现稳定和密集包装的良性大肠杆菌生物膜形成在呈现联苯甘露糖苷具有最高的fim +大肠杆菌初始附着力的表面上。这些非病原性生物膜在富含营养的溶源性肉汤中以高浓度（10（8）CFU mL（-1），比尿路感染的诊断阈值高100000倍）阻止了粪肠球菌的定居11天。 （LB）媒体。结果显示，fim +大肠杆菌83972的初始粘附性，所得生物膜的覆盖范围和长期稳定性以及它们预防病原体定殖的效率之间存在相关性。

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