Relationship between the Hydrophobicity of Dipeptides and the Michaelis-Menten Constant K_m of Their Hydrolysis by Carboxypeptidase-Y and Carboxypeptidase-A

Yoshifumi Kanosue; Satoshi Kojima; Yoshikazu Hiraga

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Relationship between the Hydrophobicity of Dipeptides and the Michaelis-Menten Constant K_m of Their Hydrolysis by Carboxypeptidase-Y and Carboxypeptidase-A

机译：肽的疏水性与羧肽酶-Y和羧肽酶-A水解的Michaelis-Menten常数K_m之间的关系

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The enzymatic hydrolysis of dipeptides by carboxypeptidase-Y and carboxypeptidase-A was investigated.In the enzymatic hydrolysis of the dipeptides,a good linear relationship(r=0.997 and 0.999)was found between the Michaelis-Menten constant(K_m)and the hydrophobicity of the substrates evaluated from relative elution volume in reversed-phase HPLC.The correlation suggests that the hydrophobicity of the C-terminal amino acid is a major factor in governing the stability of the enzyme-substrate complex.The difference in the slope of the linear-regression lines seems to reflect the degree of relative hydrophobicity of the binding pockets in carboxypeptidase-Y and carboxypeptidase-A.

机译：研究了羧肽酶-Y和羧肽酶-A对二肽的酶解作用。在二肽的酶解过程中，Michaelis-Menten常数（K_m）与疏水性之间存在良好的线性关系（r = 0.997和0.999）。相关性表明，C末端氨基酸的疏水性是控制酶-底物复合物稳定性的主要因素。回归线似乎反映了羧肽酶-Y和羧肽酶-A中结合口袋的相对疏水程度。

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《Bulletin of the Chemical Society of Japan》 |2004年第6期|共7页
作者
Yoshifumi Kanosue; Satoshi Kojima; Yoshikazu Hiraga;
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1. Relationship between the Hydrophobicity of Dipeptides and the Michaelis-Menten Constant K_m of Their Hydrolysis by Carboxypeptidase-Y and Carboxypeptidase-A [J] . Yoshifumi Kanosue, Satoshi Kojima, Yoshikazu Hiraga Bulletin of the Chemical Society of Japan . 2004,第6期

机译：肽的疏水性与羧肽酶-Y和羧肽酶-A水解的Michaelis-Menten常数K_m之间的关系
2. Relationship between the Hydrophobicity of Dipeptides and the Michaelis-Menten Constant K_m of Their Hydrolysis by Carboxypeptidase-Y and Carboxypeptidase-A [J] . Yoshifumi Kanosue, Satoshi Kojima, Yoshikazu Hiraga Bulletin of the Chemical Society of Japan . 2004,第6期

机译：肽的疏水性与羧肽酶-Y和羧肽酶-A水解的Michaelis-Menten常数K_m之间的关系
3. Kinetic Evidence for the Hydrophobic Atmosphere of the Catalytic Cavity in Carboxypeptidase Y Based on a Linear Relationship between Michaelis-Menten Constank K_m and Hydrophobicity of the Substrates [J] . Yoshifumi Kanosue, Yoshikazu Hiraga, Satoshi Kojima, Chemistry Letters . 2001,第5期

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4. The Relationship between the Hydrolysis Degree and Calcium-binding Capacity of Whey Protein by Enzymatic Hydrolysis [C] . Lina Zhao, Shaoyun Wang, Shunli Huang, International Conference on Mechanical, Industrial, and Manufacturing Technologies . 2014

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机译：铜（II）配合物介导的磷酸二酯，羧酸酯和酰胺水解的配体结构与反应性之间的关系。第一部分铜（II）配合物促进的磷酸二酯的水解。第二部分铜（II）配合物催化的羧酸酯和酰胺的水解。
6. Relationships between mucosal hydrolysis and transport of two phenylalanine dipeptides. [O] . D B Silk, J A Nicholson, Y S Kim 1976

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8. Hydrolysis Rate Constants for Enhancing Property-Reactivity Relationships [R] . Ellington, J. J. 1989

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Relationship between the Hydrophobicity of Dipeptides and the Michaelis-Menten Constant K_m of Their Hydrolysis by Carboxypeptidase-Y and Carboxypeptidase-A

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