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首页> 外文期刊>The international journal of developmental biology >Inhibitory smads and bone morphogenetic protein (BMP) modulate anterior photoreceptor cell number during planarian eye regeneration
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Inhibitory smads and bone morphogenetic protein (BMP) modulate anterior photoreceptor cell number during planarian eye regeneration

机译：抑制纹章和骨形态发生蛋白（BMP）调节涡虫眼再生过程中的前感光细胞数量

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摘要

Planarians represent an excellent model to study the processes of body axis and organ re-specification during regeneration. Previous studies have revealed a conserved role for the bone morphogenetic protein (BMP) pathway and its intracellular mediators Smad1/5/8 and Smad4 in planarian dorsoventral (DV) axis re-establishment. In an attempt to gain further insight into the role of this signalling pathway in planarians, we have isolated and functionally characterized the inhibitory Smads (I-Smads) in Schmidtea mediterranea. Two I-Smad homologues have been identified: Smed-smad6/7-1 and Smed-smad6/7-2. Expression of smad6/7-1 was detected in the parenchyma, while smad6/7-2 was found to be expressed in the central nervous system and the eyes. Neither single smad6/7-1 and smad6/7-2 nor double smad6/7-1,-2 silencing gave rise to any apparent disruption of the DV axis. However, both regenerating and intact smad6/7-2 (RNAi) planarians showed defects in eye morphogenesis and displayed small, rounded eyes that lacked the anterior subpopulation of photoreceptor cells. The number of pigment cells was also reduced in these animals at later stages of regeneration. In contrast, after low doses of Smed-bmp(RNAi), planarians regenerated larger eyes in which the anterior subpopulation of photoreceptor cells was expanded. Our results suggest that Smed-smad6/7-2 and Smed-bmp control the re-specification and maintenance of anterior photoreceptor cell number in S. mediterranea.
机译：浮游生物是研究再生过程中体轴和器官重新指定过程的绝佳模型。先前的研究揭示了骨形态发生蛋白（BMP）途径及其胞内介质Smad1 / 5/8和Smad4在平面腹背（DV）轴重建中的保守作用。为了进一步了解该信号传导途径在涡虫中的作用，我们分离了Schmidtea mediterranea中的抑制性Smads（I-Smads），并对其功能进行了表征。已经鉴定出两个I-Smad同源物：Smed-smad6 / 7-1和Smed-smad6 / 7-2。在实质中检测到smad6 / 7-1的表达，而在中枢神经系统和眼睛中发现了smad6 / 7-2的表达。单个smad6 / 7-1和smad6 / 7-2或双重smad6 / 7-1，-2沉默都不会引起DV轴的任何明显破坏。但是，再生的和完整的smad6 / 7-2（RNAi）涡虫在眼睛形态发生方面均表现出缺陷，并且眼睛圆小，缺少感光细胞的前亚群。在这些动物的再生后期阶段，色素细胞的数量也减少了。相比之下，在低剂量的Smed-bmp（RNAi）之后，涡虫再生了较大的眼睛，其中感光细胞的前亚群得以扩展。我们的结果表明，Smed-smad6 / 7-2和Smed-bmp控制着中链链球菌中前感光细胞数量的重新指定和维持。

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