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首页> 外文期刊>The international journal of developmental biology >The expression and alternative splicing of alpha-neurexins during Xenopus development
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The expression and alternative splicing of alpha-neurexins during Xenopus development

机译：非洲爪蟾发育过程中α-神经毒素的表达和选择性剪接

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The neurexins are involved in the formation and function of synapses. Each of three genes encodes alpha- and beta-neurexins. Additional diversity (particularly of alpha-neurexins) arises from alternative splicing, resulting in a large number of protein isoforms, the significance of which is currently unclear. We have analysed a neurexin expression and alternative splicing during development of the frog Xenopus laevis. Surprisingly, each a-neurexin gene is expressed in immature oocytes. During embryonic development, each Xenopus neurexin (nrxn) gene has a distinct temporal expression pattern, with expression being almost exclusively within the neuroepithelium. The spatial expression of nrxnl alpha and nrxnll alpha is similar in the developing CNS, with staining being observed in the optic cup and in dorsolateral regions of anterior neural tube, but not adjacent to the ventral midline. The pattern of nrxnlll alpha expression is more restricted, in several domains of the anterior neural tube. In the forebrain, expression was confined to an area in the ventrolateral neural tube; nrxnllla was also expressed in the hindbrain and spinal cord. By stage 32, a period when synaptogenesis occurs, nrxnllla is expressed midway along the neural tube's dorso-ventral axis in the hindbrain and anterior spinal cord, at the site of the primary interneuron column. Because of the striking diversity of neurexin isoforms, we analysed alternative splicing of Xenopus transcripts during development and found examples of alternative splice variants of each neurexin. The data demonstrate differential regulation of the a neurexins with respect to the gene temporal and spatial expression and alternative splicing.
机译：神经毒素参与突触的形成和功能。三种基因均编码α-和β-神经毒素。额外的多样性（尤其是α-神经毒素的多样性）是由其他剪接产生的，导致了大量的蛋白质同工型，目前尚不清楚其重要性。我们分析了青蛙非洲爪蟾发育过程中的神经毒素表达和选择性剪接。令人惊讶地，每个α-神经毒素基因在未成熟的卵母细胞中表达。在胚胎发育过程中，每个非洲爪蟾神经毒素（nrxn）基因都有独特的时间表达模式，几乎只在神经上皮内表达。在发育中的中枢神经系统中，nrxnl alpha和nrxnll alpha的空间表达相似，在视杯中和前神经管的背外侧区域观察到染色，但不邻近腹中线。在前神经管的多个区域中，nrxnlll alpha表达的模式受到更多限制。在前脑中，表达局限于腹侧神经管中的一个区域。 nrxnllla也表达在后脑和脊髓中。到第32阶段，即发生突触的时期，nrxnllla在中枢神经元原位在后脑和前脊髓中沿着神经管的背腹轴中途表达。由于神经毒素同工型的惊人多样性，我们在开发过程中分析了非洲爪蟾转录本的选择性剪接，并找到了每种神经毒素的替代剪接变体的实例。数据证明了神经毒素对基因的时空表达和选择性剪接的差异调节。

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