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首页> 外文期刊>The international journal of developmental biology >Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells
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Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells

机译：从骨髓间充质干细胞克隆的猪胚胎发育增强

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In the present study, we have characterized an isolated population of porcine bone marrow mesenchymal stem cells (MSCs) for multilineage commitment and compared the developmental potential of cloned embryos with porcine MSCs and fetal fibroblasts (FFs). MSCs exhibited robust alkaline phosphatase activity and later transformed into mineralized nodules following osteoinduction. Furthermore, MSCs underwent adipogenic and chondrogenic differentiation by producing lipid droplets and proteoglycans, respectively. Primary cultures of FFs from a female fetus at similar to 30 day of gestation were established. Donor cells at 3-4 passage were employed for nuclear transfer (INIT). Cell cycle analysis showed that the majority of MSCs in confluence were in the G0/G1 stage. Cumulus-oocyte complexes were matured and fertilized in vitro (IVF) as control. The cleavage rate was significantly (P < 0.05) higher in IVF than in NT embryos with MSCs and FFs (84.5 +/- 4.6% vs. 52.2 +/- 5.4% and 50.8 +/- 5.2%, respectively). However, blastocyst rates in IVF and NT embryos derived from MSCs (20.6 +/- 2.5% and 18.4 +/-.0%) did not differ, but were significantly (P < 0.05) higher than NT derived from FFs (9.5 +/- 2.1%). Total cell number and the ratio of ICM to total cells among blastocysts cloned from MSCs (34.4 +/- 5.2 and 0.38 +/- 0.08, respectively) were significantly (P < 0.05) higher than those from FFs (22.6 +/- 5.5 and 0.18 +/- 0.12, respectively). Proportions of TUNEL positive cells in NTembryosfrom FFs (7.3 +/- 1.8%) were significantly (P < 0.05) higher than in MSCs (4.6 +/- 1.3%) and IVF (2.5 +/- 0.9%). The results clearly demonstrate that multipotent bone marrow MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned porcine embryos.
机译：在本研究中，我们已经表征了猪骨髓间充质干细胞（MSCs）的分离群体的多谱系承诺，并比较了具有猪MSCs和胎儿成纤维细胞（FFs）的克隆胚胎的发育潜力。 MSC表现出强大的碱性磷酸酶活性，随后在骨诱导后转变为矿化的结节。此外，MSC分别通过产生脂质液滴和蛋白聚糖而经历脂肪形成和软骨形成分化。建立了与妊娠30天相似的雌性胎儿FFs的原代培养。将3-4代的供体细胞用于核移植（INIT）。细胞周期分析表明，汇合的大多数MSC处于G0 / G1期。卵-卵母细胞复合物成熟并在体外受精（IVF）作为对照。 IVF的卵裂率显着（P <0.05）高于具有MSC和FF的NT胚胎（分别为84.5 +/- 4.6％和52.2 +/- 5.4％和50.8 +/- 5.2％）。然而，源自MSCs的IVF和NT胚胎中的胚泡率（20.6 +/- 2.5％和18.4 +/-。0％）没有差异，但比源自FFs的NT（9.5 + / -2.1％）。从MSC克隆的囊胚中的总细胞数和ICM与总细胞的比例（分别为34.4 +/- 5.2和0.38 +/- 0.08）显着（P <0.05）高于FFs（22.6 +/- 5.5和FFs）。分别为0.18 +/- 0.12）。 FFs在NT胚胎中TUNEL阳性细胞的比例（7.3 +/- 1.8％）显着（P <0.05）高于MSCs（4.6 +/- 1.3％）和IVF（2.5 +/- 0.9％）。结果清楚地表明，多能干骨髓MSC作为供体细胞比FF具有更大的潜力，可以提高克隆的猪胚胎的产量。

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