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首页> 外文期刊>The international journal of developmental biology >Vasculogenesis and angiogenesis in the mouse embryo studied using quail/mouse chimeras
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Vasculogenesis and angiogenesis in the mouse embryo studied using quail/mouse chimeras

机译：使用鹌鹑/小鼠嵌合体研究小鼠胚胎中的血管生成和血管生成

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Using quail/chick chimeras, we have previously shown that different embryonic territories are vascularized through two distinct mecanisms, angiogenesis and vasculogenesis. Angiogenesis occurs in tissues of somatopleural origin, vasculogenesis occurs in territories of splanchnopleural origin. The aim of this work was to establish if these modes of vascularization were conserved in the mammalian embryo. Since in vivo manipulations with mammalian embryos are difficult to perform, we used a quail/mouse chimera approach. Mouse limb buds of somatopleural origin, and visceral organ rudiments of splanchnopleural origin, were grafted into the coelomic cavity of 2.5 day-old quail embryos. After four to seven days, the hosts were killed and the origin of the endothelial cells in the mouse tissues was determined by double staining with the quail endothelial and hematopoietic cell-specific marker, QH1 and mouse-specific VEGFR2 and VEGFR3 probes. Our findings show that the great majority of vessels which developed in the mouse limbs was QH1(+), indicating that these tissues were vascularized by angiogenesis. Conversely, visceral organs were vascularized through the vasculogenesis process by mouse endothelial cells which differentiated in situ. These results demonstrate for the first time that in the mouse embryo, as previously shown in avian species, the tissues from somatopleural origin are vascularized by angiogenesis, while rudiments of a splanchnopleural origin are vascularized by vasculogenesis, both at vascular and lymphatic levels.
机译：使用鹌鹑/小鸡嵌合体，我们以前已经表明，通过两种不同的机制，血管生成和血管生成，使不同的胚胎领地血管化。血管发生发生在体胸膜起源的组织中，血管发生发生在内脏胸膜起源的区域中。这项工作的目的是确定哺乳动物胚胎中这些血管化模式是否保守。由于很难对哺乳动物胚胎进行体内操作，因此我们采用了鹌鹑/小鼠嵌合体方法。将体膜胸膜起源的小鼠肢芽和内脏胸膜起源的内脏器官粗枝移植到2.5天大的鹌鹑胚胎的腔腔中。 4至7天后，将宿主杀死，并用鹌鹑内皮细胞和造血细胞特异性标记物QH1和小鼠特异性VEGFR2和VEGFR3探针进行两次染色，确定小鼠组织中内皮细胞的起源。我们的发现表明，在小鼠四肢中发育的大部分血管是QH1（+），表明这些组织通过血管生成而被血管化。相反，内脏器官通过原位分化的小鼠内皮细胞通过血管生成过程被血管化。这些结果首次证明，如先前在禽类中所示，在小鼠胚胎中，通过血管生成使来自胸膜起源的组织血管化，而通过血管生成在血管和淋巴水平上对内脏胸膜起源的残留物进行血管化。

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