...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The international journal of biochemistry and cell biology >Metabolic signatures of malignant progression in prostate epithelial cells.
【24h】

Metabolic signatures of malignant progression in prostate epithelial cells.

机译：前列腺上皮细胞恶性进展的代谢特征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prognostic markers that can distinguish indolent from aggressive prostate cancer could have substantial patient benefit, helping to target patients most in need of radical intervention, while avoiding overtreatment of a highly prevalent condition. The search for novel cancer biomarkers has been facilitated by the development of technologies for "global" biomolecular profiling, used in the sciences of transcriptomics, proteomics and metabolic profiling (metabonomics/metabolomics). Using an NMR-based approach we compared intracellular and extracellular metabolic profiles from the immortalised, non-tumourigenic prostate epithelial cell line, RWPE-1 and two tumourigenic sublines with increasing malignant phenotypes, WPE1-NB14 and WPE1-NB11, generated by N-methyl-N-nitrosourea (MNU) mutagenesis. Collectively, these cell lines present an in vitro model of prostate cancer progression and disease aggression. We observed progressive alterations to intracellular levels of multiple metabolites from choline and branched chain amino acid metabolic pathways from RWPE-1 to WPE1-NB14 to WPE1-NB11 cells. In addition specific perturbations to intracellular glycine and lactate and extracellular lactate and alanine were observed relative to the parent line. The pathways implicated by comparative metabolic profiling in this model are known to be altered in human prostate cancer, and potentially represent a source of biomarkers for prostate cancer aggression.
机译：可以区分惰性和侵袭性前列腺癌的预后标志物可以为患者带来可观的收益，帮助针对最需要根本干预的患者，同时避免过度治疗高度普遍的疾病。转录组学，蛋白质组学和代谢谱学（代谢组学/代谢组学）中使用的“全球”生物分子谱分析技术的发展促进了新型癌症生物标志物的搜索。使用基于NMR的方法，我们比较了永生的，非致瘤性前列腺上皮细胞系RWPE-1和两个致瘤性亚系的细胞内和细胞外代谢谱，它们具有由N-甲基产生的恶性表型不断增加的WPE1-NB14和WPE1-NB11 -N-亚硝基脲（MNU）诱变。这些细胞系共同提供了前列腺癌进展和疾病侵袭的体外模型。我们观察到胆碱和支链氨基酸代谢途径从RWPE-1到WPE1-NB14到WPE1-NB11细胞的多种代谢产物的细胞内水平逐渐改变。另外，相对于亲本系，观察到对细胞内甘氨酸和乳酸以及细胞外乳酸和丙氨酸的特异性扰动。已知在该模型中由比较代谢谱分析牵连的途径在人前列腺癌中被改变，并且可能代表前列腺癌侵袭的生物标志物的来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号