Propagation of Duck Plague Virus from Field Outbreaks in Embryonated Duck Eggs and Confirmation By PCR

M.K. Doley; N.N. Barman; S.K. Das; G. Rajbongshi; L.M. Barkalita

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Propagation of Duck Plague Virus from Field Outbreaks in Embryonated Duck Eggs and Confirmation By PCR

机译：野鸭暴发中的鸭瘟病毒在繁殖的鸭卵中的繁殖及PCR证实

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The DP virus could be successfully isolated in 10 days old duck embryos from the field samples. Liver and spleen tissue sample was found to be more efficient samples. Isolation of DPV from positive field samples was done in duck embryos and successive7 passages were given for adapta-tion. The infected CAM and the embryos show! extensive hemorrhages throughout the body. These changes indicate propagation of DPV in embryonated duck eggs and final confirmation of DP viral nucleic acid in CAM and liver of infected duck embryo could be easily done by PCR.

机译：可以从田间样品中成功分离出10天大的鸭胚胎中的DP病毒。发现肝和脾组织样品是更有效的样品。在鸭胚胎中从阳性田样中分离DPV，并连续进行7代传代以适应。被感染的CAM和胚胎显示出来！全身大量出血。这些变化表明DPV在有雏鸭的卵中繁殖，并且通过PCR可以很容易地最终确定被感染的雏鸭的CAM和肝脏中的DP病毒核酸。

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《The Indian Veterinary Journal》 |2014年第7期|共4页
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M.K. Doley; N.N. Barman; S.K. Das; G. Rajbongshi; L.M. Barkalita;
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中图分类动物医学（兽医学）;
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1. Propagation of Duck Plague Virus from Field Outbreaks in Embryonated Duck Eggs and Confirmation By PCR [J] . M.K. Doley, N.N. Barman, S.K. Das, The Indian Veterinary Journal . 2014,第7期

机译：野鸭暴发中的鸭瘟病毒在繁殖的鸭卵中的繁殖及PCR证实
2. Transfection of embryonated Muscovy duck eggs with a recombinant plasmid is suitable for rescue of infectious Muscovy duck parvovirus [J] . Jianye Wang, Yu Huang, Jueyi Ling, Archives of virology . 2017,第12期

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3. Characterization and pathogenicity of duck plague virus isolated from natural outbreaks in ducks of Bangladesh [J] . S Akter, MA Islam, MT Hossain, Bangladesh Journal of Veterinary Medicine . 2009,第2期

机译：从孟加拉国鸭自然暴发中分离出的鸭瘟病毒的特征和致病性
4. Application of the polymerase chain reaction (PCR) for the detection of duck plague virus in Vietnam [C] . Workshop on Control of Newcastle Disease and Duck Plague in Village Poultry . 2004

机译：聚合酶链反应（PCR）在越南鸭瘟疫病毒检测中的应用
5. Development of disinfectant efficacy assay for duck hepatitis B virus using PCR and real-time quantitative PCR. [D] . Wang, Chi-Young John. 2002

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6. Pathogenicity of duck plague and innate immune responses of the Cherry Valley ducks to duck plague virus [O] . Ning Li, Tianqi Hong, Rong Li, -1

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7. Characterization and pathogenicity of duck plague virus isolated from natural outbreaks in ducks of Bangladesh [O] . S Akter, MA Islam, MT Hossain, 1970

机译：孟加拉国鸭自然爆发中鸭瘟疫病毒的特征及致病性
8. Research and Development of Adenovirus Vaccines and Parainfluenza Virus Vaccines and Adaptation of Respiratory Syncytial Virus to Embryonated Eggs of Several Avian Species [R] . Metzgar, D. P., DeMeio, J. L. 1967

机译：腺病毒疫苗和副流感病毒疫苗的研究与开发及呼吸道合胞病毒对几种禽类胚胎的适应

Propagation of Duck Plague Virus from Field Outbreaks in Embryonated Duck Eggs and Confirmation By PCR

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