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首页> 外文期刊>The Journal of Allergy and Clinical Immunology >TGF-beta differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release by human airway smooth muscle cells.
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TGF-beta differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release by human airway smooth muscle cells.

机译：TGF-β差异性调节人呼吸道平滑肌细胞释放TH2细胞因子诱导的eotaxin和eotaxin-3。

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Background Human airway smooth muscle cells (HASMs) are involved in the pathogenesis of asthma. By producing chemokines, HASMs play a role in the inflammatory processes observed in this disease. Eotaxin, eotaxin-2, and eotaxin-3 are important chemoattractants for eosinophils, and these chemokines are expressed during different phases of the allergic reaction. T H 2 cytokines and TGF-beta can be found in increased levels in patients with asthma, and these cytokines may be involved in the regulation of chemokine expression. Objective The aim of this study was to determine the effect of T H 2 cytokines and TGF-beta on the regulation of expression of eotaxin, eotaxin-2, and eotaxin-3 by HASMs. Methods HASMs were incubated for 24 hours with IL-4, IL-13, TGF-beta1, or combinations of these cytokines. Protein and mRNA levels of eotaxin and eotaxin-3 were evaluated by sandwich ELISA and reverse transcriptase-PCR. Results IL-4 and IL-13 induced mRNA and protein for both eotaxin and eotaxin-3. Eotaxin-2 mRNA andprotein were not detected in HASMs. TGF-beta alone did not induce expression of the eotaxins. However, in combination with IL-4 or IL-13, TGF-beta enhanced eotaxin production and inhibited T H 2 cytokine-induced eotaxin-3 production. Conclusion TGF-beta differentially regulates T H 2 cytokine-induced eotaxin and eotaxin-3 release.
机译：背景技术人气道平滑肌细胞（HASM）参与哮喘的发病机理。通过产生趋化因子，HASM在该疾病中观察到的炎症过程中起作用。嗜酸性粒细胞趋化因子，嗜酸性粒细胞趋化因子2和嗜酸性粒细胞趋化因子3是嗜酸性粒细胞的重要趋化因子，这些趋化因子在变态反应的不同阶段表达。在哮喘患者中可以发现T H 2细胞因子和TGF-β水平升高，并且这些细胞因子可能与趋化因子表达的调节有关。目的本研究的目的是确定T H 2细胞因子和TGF-β对HASMs调节eotaxin，eotaxin-2和eotaxin-3表达的影响。方法将HASM与IL-4，IL-13，TGF-beta1或这些细胞因子的组合孵育24小时。通过夹心ELISA和逆转录酶-PCR评估了eotaxin和eotaxin-3的蛋白质和mRNA水平。结果IL-4和IL-13诱导了eotaxin和eotaxin-3的mRNA和蛋白。在HASM中未检测到Eotaxin-2 mRNA和蛋白。单独的TGF-β不能诱导eotaxins的表达。但是，与IL-4或IL-13结合使用时，TGF-β增强了Eotaxin的产生，并抑制了T H 2细胞因子诱导的Eotaxin-3的产生。结论TGF-β差异性调节T H 2细胞因子诱导的eotaxin和eotaxin-3的释放。

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