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首页> 外文期刊>Biochemistry (Moscow). Supplement, Series A. Membrane and cell biology >Two-Photon Excitation Spectrum of Fluorescence of the Light-Harvesting Complex B800-850 from Allochromatium minutissimum within 1200-1500(600-750) nm Spectral Range Is Not Carotenoid Mediated
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Two-Photon Excitation Spectrum of Fluorescence of the Light-Harvesting Complex B800-850 from Allochromatium minutissimum within 1200-1500(600-750) nm Spectral Range Is Not Carotenoid Mediated

机译：1200-1500（600-750）nm光谱范围内异源异色铬的光捕集络合物B800-850的荧光的双光子激发光谱不是类胡萝卜素介导的

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摘要

Two types of peripheral light-harvesting complexes LH2 (B800-850) from photosynthetic purplebacterium Allochromatium minutissimum were studied. First type containing carotenoids was prepared fromwild type cells. The other one was obtained from carotenoid depleted cells grown with diphenylamine. We haveshown that under laser femtosecond excitation within absorption 1200-1500 nm wavelength range the two-photon excitation of LH2 complexes takes place. This can be observed as fluorescence of bacteriochlorophyll(BCh1) spectral form B850 (BCh1 molecules of circular aggregate with strong exciton interaction in 850 nmspectral domain). LH2 fluorescence excitation spectra under two-photon excitation are the same for carotenoid-containing and carotenoidless preparations. In both cases the broad band with peak near 1350 (675) nm(FWHM ~ 240 (120) nm) was found. It is concluded that the broad band with peak near 1350 (675) nm in two-photon excitation spectra of LH2 complexes from Allochromatium minutissimum cannot be interpreted as two-photon excitation band of the optically forbidden S_0→S-_1transition of carotenoids (rhodopin). Possiblenature of this band is discussed.
机译：研究了来自光合紫色细菌异形异色菌的两种类型的外围光收集复合物LH2（B800-850）。从野生型细胞制备包含类胡萝卜素的第一类。另一个是从与二苯胺一起生长的类胡萝卜素耗尽的细胞中获得的。我们已经表明，在吸收波长为1200-1500 nm的激光飞秒激发下，发生了LH2络合物的双光子激发。这可以从细菌叶绿素（BCh1）光谱形式B850的荧光中观察到（BCh1分子的环状聚集体在850 nm光谱域中具有强烈的激子相互作用）。含类胡萝卜素和无类胡萝卜素的制剂在两个光子激发下的LH2荧光激发光谱相同。在这两种情况下，均发现了峰值在1350（675）nm附近的宽带（FWHM〜240（120）nm）。结论是，来自异源异色铬的LH2络合物的LH2络合物的双光子激发光谱中在1350（675）nm附近具有峰值的宽带不能解释为光学禁止的类胡萝卜素（rhodopin）S_0→S-_1跃迁的两光子激发带。 。讨论了该频段的可能性质。

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