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首页> 外文期刊>The Journal of Allergy and Clinical Immunology >Flow cytometry imaging identifies rare T(H)2 cells expressing thymic stromal lymphopoietin receptor in a 'proallergic' milieu.
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Flow cytometry imaging identifies rare T(H)2 cells expressing thymic stromal lymphopoietin receptor in a 'proallergic' milieu.

机译：流式细胞仪成像识别罕见的T（H）2细胞表达“原变”环境中的胸腺基质淋巴细胞生成素受体。

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BACKGROUND: Thymic stromal lymphopoietin (TSLP) is expressed at sites of allergic inflammation, including eczematous skin. This cytokine has been reported to exert its T(H)2-inducing properties through dendritic cells. Expression of TSLP receptor on the surface of activated T(H)2 cells could amplify T(H)2 responses at inflamed sites through the direct actions of TSLP. OBJECTIVE: To test rigorously whether T(H)2 cells induced by "proallergic" factors express TSLP receptor and characterize these cells using an experimental platform that combines flow cytometry with microscopic capabilities. METHODS: CD4(+) T cells isolated from patients with atopic dermatitis or normal healthy controls were cocultured with autologous dendritic cells in the presence of T(H)2-promoting stimuli (TSLP +/- allergen and staphylococcal enterotoxin B +/- TSLP). Surface expression of TSLP receptor was analyzed by image-based flow cytometry, and responsiveness of purified T cells to TSLP was assessed by phosphorylation of signal transducer and activator of transcription-5 and cytokine secretion. RESULTS: T(H)2-promoting stimuli induced a robust population of activated T(H)2 cells (CD25(+)IL-4(+)). Regardless of the nature of the stimulus, flow cytometry imaging confirmed that T cells expressing TSLP receptor were rare, constituting a minor fraction of the IL-4(+) T cell pool; however, TSLP responsiveness was nonetheless detectable. Analysis of cell size and nuclear morphology revealed preferential expression of TSLP receptor on IL-4-expressing cells undergoing mitosis. Analysis of lesional skin in atopic dermatitis supported the view that rare IL-4(+) T cells expressing TSLP receptor are present at inflamed sites. CONCLUSION: In a "proallergic" milieu, TSLP receptor is preferentially expressed on rare actively dividing T(H)2 cells. The direct action of TSLP on T cells could amplify T(H)2 responses at sites of allergic inflammation.
机译：背景：胸腺基质淋巴细胞生成素（TSLP）在包括皮肤湿疹在内的过敏性炎症部位表达。据报道该细胞因子通过树突状细胞发挥其诱导T（H）2的特性。 TSLP受体在活化的T（H）2细胞表面的表达可通过TSLP的直接作用放大发炎部位的T（H）2反应。目的：使用结合流式细胞仪和显微镜功能的实验平台，严格测试“前过敏”因子诱导的T（H）2细胞是否表达TSLP受体并表征这些细胞。方法：在存在T（H）2刺激（TSLP +/-变应原和葡萄球菌肠毒素B +/- TSLP）的情况下，将自特应性皮炎或正常健康对照者分离的CD4（+）T细胞与自体树突状细胞共培养。 ）。通过基于图像的流式细胞仪分析TSLP受体的表面表达，并通过信号转导和转录激活子5的磷酸化以及细胞因子的分泌来评估纯化的T细胞对TSLP的反应性。结果：促进T（H）2刺激诱导大量的活化T（H）2细胞（CD25（+）IL-4（+））。不论刺激的性质如何，流式细胞仪成像均证实表达TSLP受体的T细胞很少，仅占IL-4（+）T细胞池的一小部分。但是，仍然可以检测到TSLP响应。细胞大小和细胞核形态的分析显示TSLP受体在经历有丝分裂的IL-4表达细胞上优先表达。在特应性皮炎中病变皮肤的分析支持以下观点：发炎部位存在罕见的表达TSLP受体的IL-4（+）T细胞。结论：在“过敏原”环境中，TSLP受体优先在罕见的活跃分裂的T（H）2细胞上表达。 TSLP对T细胞的直接作用可能会放大过敏性发炎部位的T（H）2反应。

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