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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >PATTERNS OF OSCILLATION DURING MITOSIS IN PLASMODIA OF PHYSARUM POLYCEPHALUM
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PATTERNS OF OSCILLATION DURING MITOSIS IN PLASMODIA OF PHYSARUM POLYCEPHALUM

机译：聚乙烯多孢子虫血浆有丝分裂期间的振荡模式

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The rhythmic contraction pattern in plasmodia of Physarum polycephalum was studied to determine whether characteristic changes occur during the synchronized nuclear division. An electrical method that measures the contraction rhythm in situ during several cell cycles was used. Biopsies of the plasmodia were taken at 17 min intervals for precise determination of the cell cycle stages and were correlated with the simultaneously measured contraction rhythm. All measurements were performed in a temperature controlled environment (27 degrees C) at 100% relative humidity with the plasmodia (less than 24 h old) growing on a semi-defined agar medium. A total of 14 different plasmodia have been examined, and on one occasion the plasmodium was followed through 3 subsequent mitoses. The mitotic stages were identified with aceto-orcein coloring techniques and by fluorescence methods. Except for a few cases where a mitotic asynchrony of 2-3 min was observed, the mitotic events occurred simultaneously in the nuclei within a single plasmodium. Both the occurrence of the first mitosis after inoculation and the intermitotic times were highly variable. Our study indicates that the contraction rhythm in plasmodia of Physarum is unperturbed during the synchronized nuclear division. However, in 5 of the 17 examined mitoses an amplitude decay was observed. We discuss possible explanations for the obtained results with emphasis on the applied techniques, interpretation of the oscillation patterns, and possible restrictions in the cell itself. [References: 39]
机译：研究了多头浆虫的节律性收缩模式，以确定在同步核分裂过程中是否发生特征性变化。使用了一种在多个细胞周期中原位测量收缩节律的电学方法。以17分钟的间隔进行浆膜活检，以精确确定细胞周期阶段，并与同时测量的收缩节律相关。所有测量均在温度控制的环境（27摄氏度）中，相对湿度为100％的条件下进行，疟原虫（小于24小时龄）生长在半确定的琼脂培养基上。总共检查了14种不同的疟原虫，一次通过3次随后的有丝分裂追踪了疟原虫。有丝分裂阶段用乙酰-大黄素着色技术和荧光方法鉴定。除了少数情况下观察到2-3分钟的有丝分裂异步外，有丝分裂事件同时发生在单个疟原虫的细胞核中。接种后第一次有丝分裂的发生和间断时间均高度可变。我们的研究表明，在同步核分裂过程中，浆虫的收缩节律不受干扰。但是，在检查的17个有丝分裂中有5个观察到幅度衰减。我们将重点讨论应用技术，振荡模式的解释以及单元本身可能的限制，从而对获得的结果进行可能的解释。 [参考：39]

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