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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >PEROXIDASE AND NOT LACCASE IS THE ENZYME RESPONSIBLE FOR CELL WALL LIGNIFICATION IN THE SECONDARY THICKENING OF XYLEM VESSELS IN LUPINUS
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PEROXIDASE AND NOT LACCASE IS THE ENZYME RESPONSIBLE FOR CELL WALL LIGNIFICATION IN THE SECONDARY THICKENING OF XYLEM VESSELS IN LUPINUS

机译:过氧化物酶而不是乳糖酶是羽扇豆二倍体血管二次增厚过程中负责细胞壁木质化的酶

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摘要

The post-exponential growth phase of lupin (Lupinus albus cv. Multolupa) hypocotyls is characterized by a strong deposition of lignins in the primary and secondary walls of the xylem vessels. Coinciding with this phenomenon, there is a clearly peroxidatic activity in both the primary cell walls and the outer-most layers of the secondary thickening of the xylem vessels, as demonstrated by 3,3'-diaminobenzidine cytochemistry. This activity was completely inhibited by KCN and the removal of H2O2 and was not due to laccase since this enzyme shows an almost total inability to oxidize 3,3'-diaminobenzidine both in the presence and in the absence of H2O2 The absence of laccase-like activities in cell walls of vascular cells was supported by the fact that cell wall proteins from vascular cells were only capable of oxidizing 3,3'-diaminobenzidine and coniferyl alcohol in the presence of H2O2 These results support the idea of an exclusive role of peroxidase (and exclude any role for laccase) in lignin formation in the secondary thickening of xylem vessels in Lupinus. [References: 23]
机译:羽扇豆(Lupinus albus cv。Multolupa)下胚轴的指数后生长阶段的特征在于木质素在木质部血管的第一壁和第二壁中的强烈沉积。与此现象相吻合的是,木质部血管的第二次增厚的原代细胞壁和最外层均具有明显的过氧化活性,如3,3'-二氨基联苯胺细胞化学所证实。该活性被KCN和H2O2的去除完全抑制了,并且不是由于漆酶引起的,因为在存在和不存在H2O2的情况下,该酶几乎都无法氧化3,3'-二氨基联苯胺。血管细胞壁蛋白在H2O2存在下仅能氧化3,3'-二氨基联苯胺和松柏醇的事实支持了血管细胞壁活性的变化。这些结果支持了过氧化物酶(并排除漆酶的作用)在羽扇豆木质部血管的二次增厚中木质素的形成。 [参考:23]

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