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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Membrane recycling occurs during asymmetric tip growth and cell plate formation in Fucus distichus zygotes
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Membrane recycling occurs during asymmetric tip growth and cell plate formation in Fucus distichus zygotes

机译：膜回收发生在不动的顶生合子不对称尖端生长和细胞板形成过程中。

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摘要

Zygotes of the brown alga Fucus distichus undergo a series of intracellular changes resulting in the establishment of a polar growth axis prior to the first embryonic cell division. In order to examine the dynamics of membrane recycling which occur in the zygote during polar growth of the rhizoid, we probed living Fucus zygotes with the vital stain FM4-64, N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyri dinium dibromide. In newly fertilized, spherical zygotes, FM4-64 staining is symmetric and predominantly in the perinuclear region which is rich in endoplasmic reticulum, Golgi, and vacuolar membranes. As rhizoid or tip growth is initiated, this population of stained membranes becomes asymmetrically redistributed, concentrating at the rhizoid tip and extending centrally to the perinuclear region. This asymmetric localization is maintained in the zygote throughout polar growth of the rhizoid and during karyokinesis. Subsequently FM4-64 staining also begins to accumulate in a central location between the daughter nuclei. As cytokinesis proceeds, this region of stain expands laterally from this central location, perpendicular to the plane of polar rhizoid outgrowth. The staining pattern thus delineates the formation of a cell plate, similar spatially to the accumulation of nascent plate membranes of higher plants. Treatment of Fucus zygotes with brefeldin-A inhibits both asymmetric growth of the rhizoid and formation of a new cell plate. These data suggest that in F. distichus FM4-64 is labeling a Golgi-derived membrane fraction that appears to be recycling between the site of tip growth, perinuclear region, and new cell plate. [References: 55]
机译：褐藻褐藻（Fusus distichus）的合子经历了一系列细胞内变化，导致在第一次胚胎细胞分裂之前建立了一个极地生长轴。为了检查在根状茎的极性生长过程中合子中发生的膜循环动力学，我们用活体染色剂FM4-64，N-（3-三乙基铵丙基）-4-（6-（4- （二乙氨基）苯基）六三烯基）吡啶二溴化二铵。在新受精的球形合子中，FM4-64染色是对称的，并且主要分布在核内区域，该区域富含内质网，高尔基体和液泡膜。当根状或尖端生长开始时，这组染色的膜就变得不对称地重新分布，集中在根状的尖端并中心延伸至核周区域。这种不对称的定位在整个根状体的极性生长期间以及在核运动期间都保持在合子中。随后，FM4-64染色也开始积累在子核之间的中心位置。随着胞质分裂的进行，该污渍区域从该中心位置横向扩展，垂直于极性根状茎生长的平面。因此，染色模式描绘了细胞平板的形成，在空间上与高等植物的新生平板膜的积累相似。用布雷菲德菌素-A处理岩藻合子抑制了根状茎的不对称生长和新细胞板的形成。这些数据表明，在F. distichus中，FM4-64标记了高尔基体来源的膜部分，该部分似乎在尖端生长部位，核周区域和新细胞板之间循环。 [参考：55]

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