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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Isolation of the ftsZ gene from plastid-deficient generative cells of Lilium longiflorum
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Isolation of the ftsZ gene from plastid-deficient generative cells of Lilium longiflorum

机译：从长百合的质体缺陷型生殖细胞中分离ftsZ基因

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摘要

In angiosperms, the pollen generative cell is the progenitor of the male gametes. In order to clone novel genes that are expressed specifically in male gametic cells, a simplified differential-display method with total RNA was applied to isolated generative cells of Lilium longiflorum. At least three fragments were specifically amplified in generative cells in comparison with pollen and leaf cells. Northern blot analysis showed that one of these, which was named AP6-E, was part of a gene expressed strongly in generative cells. Reverse-transcriptase polymerase chain reaction analysis also revealed that the AP6-E gene was expressed during microsporogenesis and microspore development, and its expression was gradually reduced with pollen development. However, in situ hybridization demonstrated that transcripts of the AP6-E gene preferentially accumulated in the generative cell of nearly mature pollen. The isolation of AP6-E cDNA including an open reading frame of 468 amino acids showed that the AP6-E protein is a homolog of the plant plastid division protein FtsZ. This is the first ftsZ gene isolated from a monocotyledonous plant, and it suggests that ftsZ genes have a function other than plastid division, since generative cells contain no plastids. [References: 32]
机译：在被子植物中，花粉生成细胞是雄配子的祖细胞。为了克隆在雄性配子细胞中特异性表达的新基因，将具有总RNA的简化的差异显示方法应用于离体的长百合百合生殖细胞。与花粉和叶细胞相比，在生殖细胞中至少扩增了三个片段。 Northern印迹分析表明，其中一个被称为AP6-E，是在生殖细胞中强烈表达的基因的一部分。逆转录酶聚合酶链反应分析还表明，AP6-E基因在小孢子发生和小孢子发育过程中表达，其表达随着花粉的发育而逐渐减少。然而，原位杂交表明AP6-E基因的转录本优先积累在接近成熟花粉的生殖细胞中。包含468个氨基酸的开放阅读框的AP6-E cDNA的分离表明，AP6-E蛋白是植物质体分裂蛋白FtsZ的同源物。这是从单子叶植物中分离出的第一个ftsZ基因，这表明ftsZ基因除了质体分裂外还具有其他功能，因为生殖细胞不含质体。 [参考：32]

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