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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Ultrastructure of the host-pathogen interface in daylily leaves infected by the rust fungus Puccinia hemerocallidis
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Ultrastructure of the host-pathogen interface in daylily leaves infected by the rust fungus Puccinia hemerocallidis

机译：黄锈病叶片锈病真菌Puccinia hemerocallidis感染宿主-病原体界面的超微结构

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Transmission electron microscopy was used to examine details of the host-pathogen interface in daylily leaf cells infected by the rust fungus Puccinia hemerocallidis. Samples were prepared for study by high-pressure freezing followed by freeze substitution. The outstanding preservation of ultrastructural details afforded by this fixation protocol greatly facilitated the study of this host-pathogen interface. The extrahaustorial membrane that separated each dikaryotic haustorium from the cytoplasm of its host cell was especially well preserved and appeared almost completely smooth in profile. Large aggregations of tubular cytoplasmic elements were present near haustoria in infected host cells. Many of these tubular elements were found to be continuous with the extrahaustorial membrane and conspicuous electron-dense deposits present in the extrahaustorial matrix extended into these elements. The use of gold-conjugated wheat germ agglutinin for labeling of chitin revealed that these deposits were not part of the haustorial wall. Portions of many of the tubular elements associated with haustoria were conspicuously beaded in appearance. Some tubular elements were found to be continuous with flattened cisternae that in turn bore short beaded chains. Distinctive tubular-vesicular complexes previously reported only in cryofixed rust haustoria also were found in the haustoria of R hemerocallidis. [References: 24]
机译：使用透射电子显微镜检查被锈菌Puccinia hemerocallidis感染的黄花菜叶细胞中宿主-病原体界面的细节。通过高压冷冻然后冷冻替代制备样品以供研究。该固定方案所提供的超微结构细节的出色保存，极大地促进了该宿主-病原体界面的研究。分离每个双核吸虫与其宿主细胞的细胞质的胃外膜特别好保存，并且轮廓几乎完全光滑。在被感染的宿主细胞中，靠近吸精管的肾小管胞质元件大量聚集。发现许多这些管状元件与胃外膜连续，并且存在于胃外基质中的明显的电子致密沉积物扩展到这些元件中。使用金缀合的小麦胚芽凝集素标记几丁质后发现，这些沉积物不是胃壁的一部分。与haustoria相关的许多管状元件的部分在外观上明显串珠。发现一些管状元件与扁平的水箱连续，而水箱又带有短的串珠链。以前仅在冷冻固定的锈质吸油管中报道过的独特的管状-水泡复合物也在R hemerocallidis的吸油管中被发现。 [参考：24]

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