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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Jasplakinolide reversibly disrupts actin filaments in suspension-cultured tobacco BY-2 cells
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Jasplakinolide reversibly disrupts actin filaments in suspension-cultured tobacco BY-2 cells

机译：茉莉素可逆可逆地破坏悬浮培养的烟草BY-2细胞中的肌动蛋白丝

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摘要

Jasplakinolide is potentially a useful pharmacological tool for the study of actin organization and dynamics in living cells, since it induces actin polymerization in vitro and. unlike phalloidin, is membrane permeative. In the present work, the effect of jasplakinolide on the actin cytoskeleton of living suspension-cultured Nicotiana tabacum 'Bright Yellow 2' cells was investigated. Actin filaments in the living cells were disrupted by jasplakinolide. The effect of jasplakionlide on the actin cytoskeleton was concentration and time dependent. When cells were treated with a moderate concentration (150 nM) of jasplakinolide, cortical actin filaments were disrupted preferentially, whereas actin aggregated at the perinuclear region. With concentrations higher than 400 nM and exposure times longer than 30 min. actin filaments in the cell disappeared completely. The effect of jasplakinolide on the actin cytoskeleton was reversible even at high concentration. Actin bundles appeared first in the perinuclear region within 5 min, and the cortical actin array was reestablished in 15 min, suggesting that actin filaments might be organized at this region. [References: 20]
机译：Jasplakinolide可能是研究活细胞肌动蛋白组织和动力学的有用药理学工具，因为它在体外和体外诱导肌动蛋白聚合。与鬼笔环肽不同，它是膜渗透性的。在本工作中，研究了茉莉素醇内酯对悬浮培养的烟草“亮黄2”细胞肌动蛋白细胞骨架的影响。 jasplakinolide破坏了活细胞中的肌动蛋白丝。茉莉酸内酯对肌动蛋白细胞骨架的影响是浓度和时间依赖性的。当用中等浓度（150 nM）的jasplakinolide处理细胞时，皮质肌动蛋白丝被优先破坏，而肌动蛋白聚集在核周区域。浓度高于400 nM，暴露时间超过30分钟。细胞中的肌动蛋白丝完全消失。即使在高浓度下，jasplakinolide对肌动蛋白细胞骨架的作用也是可逆的。肌动蛋白束首先出现在5分钟内的核周区域，皮质肌动蛋白阵列在15分钟内重新建立，表明肌动蛋白丝可能在该区域组织。 [参考：20]

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