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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Localization of calcium in the pericarp cells of tomato fruits during the development of blossom-end rot
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Localization of calcium in the pericarp cells of tomato fruits during the development of blossom-end rot

机译:开花期腐烂发生过程中番茄果实果皮细胞中钙的定位

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Blossom-end rot (BER) of tomato (Lycopersicon esculentum) fruits is considered to be a physiological disorder caused by calcium deficiency. We attempted to clarify the localization of calcium in the pericarp cells and the ultrastructural changes during the development of BER. Calcium precipitates were observed as electron-dense deposits by an antimonate precipitation method. Some calcium precipitates were localized in the cytosol, nucleus, plastids, and vacuoles at an early developmental stage of normal fruits. Calcium precipitates were increased markedly on the plasma membrane during the rapid-fruit-growth stage compared with their level at the early stage. Cell collapse occurred in the water-soaked region at the rapid-fruit-growth stage in BER fruits. There were no visible calcium precipitates on the traces of plasma membrane near the cell wall of the collapsed cells. The amount of calcium precipitates on plasma membranes near collapsed cells was smaller than that in the cells of normal fruits and normal parts of BER fruits, and the amount on cells near collapsed cells was small. The amount of calcium precipitates on the plasma membranes increased as the distance from collapsed cells increased. On the other hand, calcium precipitates were visible normally in the cytosol, organelles, and vacuoles and even traces of them in collapsed cells. The distribution pattern of the calcium precipitates on the plasma membrane was thus considerably different between normal and BER fruits. On the basis of these observations, we concluded that calcium deficiency in plasma membranes caused cell collapses in BER tomato fruits. [References: 34]
机译:番茄(Lycopersicon esculentum)果实的花期腐烂(BER)被认为是由钙缺乏引起的一种生理疾病。我们试图阐明钙在果皮细胞中的定位以及在BER发生过程中的超微结构变化。通过锑酸盐沉淀法观察到钙沉淀物为电子致密沉积物。在正常果实的早期发育阶段,一些钙沉淀物位于胞质,细胞核,质体和液泡中。与果实早期相比,在果实快速生长阶段,钙膜上的钙沉淀明显增加。在BER水果的速生阶段,浸水区域发生了细胞崩溃。在塌陷细胞的细胞壁附近的质膜上没有可见的钙沉淀。塌陷细胞附近的质膜上的钙沉淀量少于正常果实和BER果实正常部位的细胞中的钙沉淀,塌陷细胞附近的细胞上的钙沉淀的量较小。随着与塌陷细胞的距离增加,钙沉淀在质膜上的数量增加。另一方面,钙沉淀在细胞质,细胞器和液泡中正常可见,甚至在塌陷的细胞中也有痕量。因此,钙沉淀物在质膜上的分布模式在正常和BER水果之间有很大不同。基于这些观察,我们得出结论,质膜缺乏钙会导致BER番茄果实中的细胞崩溃。 [参考:34]

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