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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Multiple activities of a novel substance, dictyopyrone C isolated from Dictyostelium discoideum, in cellular growth and differentiation
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Multiple activities of a novel substance, dictyopyrone C isolated from Dictyostelium discoideum, in cellular growth and differentiation

机译：从Disctyostelium discoideum分离出的一种新物质，dictyopyrone C在细胞生长和分化中的多种活性

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We report that a novel substance named dictyopyrone C (DPC) has remarkable effects on growth and differentiation of Dictyostelium discoideum Ax-2 cells, in a dose-dependent manner. In the presence of 3-15 muM DPC, differentiation of starving Ax-2 (clone MS) cells was greatly enhanced in submerged culture, when vegetative MS cells were harvested at the mid-late-exponential growth phase (>3 x 10(6) cells per ml) and starved. In contrast, DPC above 30 muM markedly impaired the progression of differentiation including cell aggregation, most of starved cells being round after 3-4 h of DPC application and then lysed during further incubation. In the presence of 30 muM DPC however, MS cells that had been harvested at the early exponential growth phase (<5 x 10(5) cells per ml) and starved became neither round nor lysed and exhibited rather enhanced differentiation. Essentially the same results were obtained in cultures of starved cells on nonnutrient agar. With respect to the DPC effect on MS cells growing in axenic medium, cell lysis and growth inhibition by DPC at concentrations higher than 15 muM were realized in the mid-late-exponential-growth-phase cells (>3 x 10(6) cells per ml) but not in the early-exponential-growth-phase cells (<5 x 10(5) cells per ml). Moreover, analysis using synchronized MS cells has demonstrated that the DPC effect changes in a cell-cycle-dependent manner. In contrast to such unique DPC actions, the pyrone ring of DPC had no effects on growth and differentiation within the range of 3-120 muM tested. These findings strongly suggested the importance of the combined structure of the pyrone ring and the linear carbon chain in revelation of the DPC activities. [References: 26]
机译：我们报告称一种名为dictyopyrone C（DPC）的新物质对Disctyostelium discoideum Ax-2细胞的生长和分化具有显着影响，且呈剂量依赖性。在3-15μMDPC的存在下，当在中晚期指数生长期（> 3 x 10（6）收集营养MS细胞时，在水下培养中饥饿的Ax-2（克隆MS）细胞的分化会大大增强）细胞/毫升）并饿死。相反，高于30μM的DPC明显损害了分化的进程，包括细胞聚集，大多数饥饿的细胞在DPC施用3-4小时后呈圆形，然后在进一步温育中裂解。但是，在存在30μMDPC的情况下，在早期指数生长期收获的MS细胞（每毫升<5 x 10（5）个细胞）并且饥饿后既没有圆形也没有裂解，并且表现出相当强的分化能力。在非营养琼脂上的饥饿细胞培养中，基本上获得了相同的结果。关于DPC对在无菌培养基中生长的MS细胞的影响，在中晚期指数生长相细胞（> 3 x 10（6））中实现了浓度高于15μM时DPC对细胞的溶解和生长抑制每毫升），但在早期指数生长阶段的细胞中则没有（每毫升<5 x 10（5）个细胞）。此外，使用同步MS单元进行的分析表明，DPC效应以依赖细胞周期的方式变化。与这种独特的DPC作用相反，在测试的3-120μM范围内，DPC的吡喃环对生长和分化没有影响。这些发现强烈表明，在揭示DPC活性中，吡咯环和线性碳链的结合结构的重要性。 [参考：26]

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