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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Ultrastructural localization of glutathione in Cucurbita pepo plants
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Ultrastructural localization of glutathione in Cucurbita pepo plants

机译：南瓜属植物中谷胱甘肽的超微结构定位

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摘要

Electronmicroscopic immunogold cytochemistry was used to investigate the cellular and subcellular distribution of glutathione in root and leaf cells of Styrian pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) plants. Gold particles bound to glutathione were found in various cell structures. Statistical evaluation of the gold particle density was made for different cell compartments including nuclei, mitochondria. plastids, peroxisomes, and the cytosol. In each cell type the highest level of glutathione immunoreactivity occurred in mitochondria, for which the labeling density was found to be higher in mesophyll cells of the youngest fully developed leaves (younger leaves) than in the 5th leaves (older leaves) or in root tip cells. Additionally, a statistically significant increase of gold particles bound to glutathione was observed in nuclei (22%) and the cytosol (14%) of the root cells in comparison with mesophyll cells of older (17% and 9%, respectively) and younger leaves (11% and 6%, respectively). The relevance and specificity of glutathione labeling is discussed with respect to difficulties of immunolocalization of low-molecular-weight compounds.
机译：用电子显微免疫金细胞化学技术研究了谷胱甘肽在施蒂利亚南瓜（南瓜属植物Pepo var。styriaca Greb。）植物的根和叶细胞中的细胞和亚细胞分布。在各种细胞结构中发现了与谷胱甘肽结合的金颗粒。对包括细胞核，线粒体在内的不同细胞室进行了金颗粒密度的统计评估。质体，过氧化物酶体和胞质溶胶。在每种细胞类型中，线粒体中谷胱甘肽的免疫反应水平最高，发现其在最年轻的完全发育叶片（幼叶）的叶肉细胞中的标记密度高于第五叶片（较老叶片）或根尖中的标记密度。细胞。此外，与较老的叶肉细胞（分别为17％和9％）和较年轻的叶肉细胞相比，在根细胞的细胞核（22％）和胞浆（14％）中观察到与谷胱甘肽结合的金颗粒的统计学显着增加。 （分别为11％和6％）。关于低分子量化合物的免疫定位困难，讨论了谷胱甘肽标记的相关性和特异性。

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