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首页> 外文期刊>Archives of virology >Artificial elevation of glutathione affects symptom development in ZYMV-infected Cucurbita pepo L. plants.
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Artificial elevation of glutathione affects symptom development in ZYMV-infected Cucurbita pepo L. plants.

机译：谷胱甘肽的人工升高会影响ZYMV感染的南瓜属植物的症状发展。

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Styrian oil pumpkin seedlings (Cucurbita pepo L. subsp. pepo var. styriaca GREB: .) were treated for 48 h with 1 mM OTC (L-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. They were inoculated with zucchini yellow mosaic virus (ZYMV) 10 days later. The effects of OTC treatment and ZYMV infection on glutathione levels were examined at the subcellular level by immunogold labeling of glutathione using a transmission electron microscope (TEM). These effects were further tested at the whole-tissue level by high performance liquid chromatography (HPLC). Such tests were carried out a) on roots, cotyledons and the first true leaves immediately after OTC treatment in order to analyze to which extent OTC increases glutathione levels in different cell compartments as well as in the whole organ; and b) in older and younger leaves and in roots three weeks after ZYMV inoculation in order to study how possible effects of OTC on symptom development would correlate with glutathione levels at the subcellular level and in the whole organ.Immunocytological and biochemical investigations revealed that, 48 h after OTC treatment, glutathione content had increased in all investigated organs, up to 144% in peroxisomes of cotyledons. Three weeks after ZYMV inoculation, glutathione labeling density had significantly increased within intact cells of infected leaves, up to 124% in the cytosol of younger leaves. Roots showed decreased amounts of glutathione in the TEM. Biochemical studies revealed that OTC treatment resulted in 41 and 51% higher glutathione content in older and younger ZYMV-infected leaves, respectively, in comparison to untreated and ZYMV-infected plants. Evaluation of symptom development at this point revealed that all untreated ZYMV-infected plants had symptoms, whereas only 42% of OTC-treated ZYMV-infected plants showed signs of symptoms. Quantification of ZYMV particles revealed that all organs of OTC-treated and ZYMV-infected plants contained significantly decreased amounts of ZYMV particles over a period of five weeks when compared to the same organs of untreated ZYMV-infected plants. We can conclude that OTC treatment and subsequently elevated glutathione contents within Styrian oil pumpkin plants led to a strong decrease in virus content, which was accompanied by a suppression of ZYMV-induced symptoms as well as reduced and delayed symptom development within plants exhibiting symptoms.
机译：用1 mM OTC（L-2-oxothiazolidine-4-羧酸）处理施蒂兰油南瓜幼苗（Cucurbita pepo L. subsp。pepo var。styriaca GREB：。）48小时，以人工增加细胞内谷胱甘肽的含量。 10天后，用西葫芦黄色花叶病毒（ZYMV）接种它们。通过使用透射电子显微镜（TEM）对谷胱甘肽进行免疫金标记，在亚细胞水平上检查了OTC处理和ZYMV感染对谷胱甘肽水平的影响。通过高效液相色谱（HPLC）在全组织水平上进一步测试了这些作用。 a）在OTC处理后立即对根，子叶和第一片真叶进行测试，以分析OTC在何种程度上增加了不同细胞区室以及整个器官中的谷胱甘肽水平; b）在接种ZYMV后三周内，在较老和较年轻的叶子和根中，研究OTC对症状发展的可能影响如何与亚细胞水平和整个器官中的谷胱甘肽水平相关。免疫细胞和生化研究表明， OTC处理后48小时，所有被调查器官的谷胱甘肽含量均增加，子叶过氧化物酶体中的含量高达144％。 ZYMV接种三周后，感染叶片完整细胞内的谷胱甘肽标记密度显着增加，年轻叶片的细胞质中谷胱甘肽标记密度高达124％。根在TEM中显示出减少的谷胱甘肽量。生化研究表明，与未经处理和经ZYMV感染的植物相比，OTC处理可使ZYMV感染的较老和较年轻的叶片中谷胱甘肽含量分别增加41％和51％。此时对症状发展的评估表明，所有未处理的ZYMV感染植物均具有症状，而仅OTC处理的ZYMV感染植物中只有42％出现症状迹象。 ZYMV颗粒的定量显示，与未经处理的ZYMV感染的植物的相同器官相比，经OTC处理和ZYMV感染的植物的所有器官在五周内的ZYMV颗粒量均显着减少。我们可以得出结论，在OTC处理以及随后在施蒂里亚油南瓜植物中增加的谷胱甘肽含量导致病毒含量大幅降低，同时抑制ZYMV诱导的症状以及在表现出症状的植物中减少和延迟症状的发展。

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