Bromodeoxyuridine DNA fiber technology in plants: replication origins and DNA synthesis in tobacco BY-2 cells under prolonged treatment with aphidicolin

Quelo AH; Verbelen JP

首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Bromodeoxyuridine DNA fiber technology in plants: replication origins and DNA synthesis in tobacco BY-2 cells under prolonged treatment with aphidicolin

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Bromodeoxyuridine DNA fiber technology in plants: replication origins and DNA synthesis in tobacco BY-2 cells under prolonged treatment with aphidicolin

机译：植物中的溴脱氧尿苷DNA纤维技术：经过蚜虫碱长时间处理的烟草BY-2细胞的复制起点和DNA合成

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Newly synthesized DNA can be observed on chromosomes or extended chromatin fibers after incorporation and immunodetection of bromodeoxyuridine. This technique, frequently used in animal cells, was adapted for use in BY-2 cells. For the first time, the origins of replication in plant cells could be visualized and monitored on DNA fibers without the use of radioactive traces. The replicon size for BY-2 cells was estimated to be 12.9 mum: and the fork rate, 1.17 mum/h. These values are comparable to those reported for tomato and mustard cells. Furthermore, the data confirm our previous observation that DNA synthesis is not totally blocked by aphidicolin. Bromodeoxyuridine incorporation into DNA was obvious from 24 h onwards after treatment with aphidicolin.

机译：掺入溴脱氧尿嘧啶核苷并对其进行免疫检测后，可以在染色体或延伸的染色质纤维上观察到新合成的DNA。该技术常用于动物细胞中，适用于BY-2细胞。首次可以在不使用放射性痕迹的情况下，在DNA纤维上显示和监视植物细胞中复制的起点。 BY-2细胞的复制子大小估计为12.9微米：分叉速率为1.17微米/小时。这些值与报道的番茄和芥末细胞的值相当。此外，数据证实了我们先前的观察结果，即蚜虫双酚未完全阻止DNA合成。从蚜虫碱处理24小时起，溴脱氧尿苷掺入DNA是明显的。

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《Protoplasma: An International Journal of Cell Biology》 |2004年第4期|共6页
作者
Quelo AH; Verbelen JP;
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正文语种 eng
中图分类细胞形态学;
关键词
tobacco BY-2 cell; aphidicolin; bromodeoxyuridine; DNA fiber; replication origin; fluorescence microscopy; POLYMERASE-ALPHA; REPLICON SIZE; 5-BROMODEOXYURIDINE; CYTOKININS; ACTIVATION;

机译：烟草BY-2细胞;蚜虫蛋白;溴脱氧尿苷;DNA纤维;复制起点;荧光显微镜;聚合酶-α;复制大小;5-溴脱氧尿嘧啶;细胞分裂素;活化;

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专利

1. Bromodeoxyuridine DNA fiber technology in plants: replication origins and DNA synthesis in tobacco BY-2 cells under prolonged treatment with aphidicolin [J] . A.-H. Quélo, J.-P. Verbelen Protoplasma . 2004,第2a4期

机译：植物中的溴脱氧尿苷DNA纤维技术：经过蚜虫碱长时间处理的烟草BY-2细胞的复制起点和DNA合成
2. NtPolI-like1 and NtPolI-like2, Bacterial DNA Polymerase I Homologs Isolated from BY-2 Cultured Tobacco Cells, Encode DNA Polymerases Engaged in DNA Replication in Both Plastids and Mitochondria [J] . Yuriko Ono, Atsushi Sakai, Katsuaki Takechi, Plant and Cell Physiology . 2007,第12期

机译：NtPolI-like1和NtPolI-like2，从BY-2培养的烟草细胞中分离出的细菌DNA聚合酶I同源物，编码参与质体和线粒体DNA复制的DNA聚合酶。
3. NtPolI-like1 and NtPolI-like2, bacterial DNA polymerase I homologs isolated from BY-2 cultured tobacco cells, encode DNA polymerases engaged in DNA replication in both plastids and mitochondria [J] . Ono Y, Sakai A, Takechi K, Plant and cell physiology . 2007,第12期

机译：从BY-2培养的烟草细胞中分离出的细菌DNA聚合酶I同源物NtPolI-like1和NtPolI-like2编码参与质体和线粒体DNA复制的DNA聚合酶
4. Abiotic tolerance of tobacco plants transformed with DnaK from a halotolerant cyanobacterium Aphanothece halophytica [C] . T Hibino, K Ono, Y Tanaka, International Congress on Photosynthesis . 2001

机译：用嗜烟草中的烟草植物转化的烟草植物的非生物耐受性来自嗜菌嗜嗜嗜菌嗜睡症
5. DNA Replication in Plants Origins of DNA Replication and Post-translational Histone Modifications During the DNA Synthesis Phase of Arabidopsis thaliana [D] . Franco Ruiz, Mariana 2012

机译：植物中的DNA复制拟南芥DNA合成阶段中DNA复制和翻译后组蛋白修饰的起源。
6. Initiation of simian virus 40 DNA replication in vitro: aphidicolin causes accumulation of early-replicating intermediates and allows determination of the initial direction of DNA synthesis. [O] . R S Decker, M Yamaguchi, R Possenti, 1986

机译：猿猴病毒40体外DNA复制的启动：蚜虫碱引起早期复制中间体的积累并可以确定DNA合成的初始方向。
7. Initiation of simian virus 40 DNA replication in vitro: aphidicolin causes accumulation of early-replicating intermediates and allows determination of the initial direction of DNA synthesis. [O] . Decker, R S, Yamaguchi, M, Possenti, R, 1986

机译：猿猴病毒40体外DNA复制的启动：蚜虫碱引起早期复制中间体的积累，并可以确定DNA合成的初始方向。
8. Structure-Activity Study of Paracetamol Analogues: Inhibition of Replicative DNASynthesis in V79 Chinese Hamster Cells [R] . Richard, A. M., Hongslo, J. K., Boone, P. F., 1991

机译：对乙酰氨基酚类似物的结构 - 活性研究：V79中国仓鼠细胞中复制DNa合成的抑制

Bromodeoxyuridine DNA fiber technology in plants: replication origins and DNA synthesis in tobacco BY-2 cells under prolonged treatment with aphidicolin

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