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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Flow cytometric studies of the host-regulated cell cycle in algae symbiotic with green paramecium
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Flow cytometric studies of the host-regulated cell cycle in algae symbiotic with green paramecium

机译：流式细胞术研究寄主调控绿色草履虫的藻类共生细胞周期

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摘要

Paramecium bursaria (green paramecium) possesses endosymbiotically growing chlorella-like green algae. An aposymbiotic cell line of P bursaria (MBw-1) was prepared from the green MB-1 strain with the herbicide paraquat. The SA-2 clone of symbiotic algae was employed to reinfect MBw-1 cells and thus a regreened cell line (MBr-1) was obtained. The regeened paramecia were used to study the impact of the host's growth status on the life cycle of the symbiotic algae. Firstly, the relationship between the tinting of algal propagation and the host cell division was investigated by counting the algal cells in single host cells during and after the host cell division and also in the stationary phase. Secondly, the changes in the endogenous chlorophyll level, DNA content, and cell size in the symbiotic algae were monitored by flow cytometry and fluorescence microscopy. The number of algae was shown to be doubled prior to or during the host cell division and the algal population in the two daughter cells is maintained at constant level until the host cell cycle reenters the cytokinesis, suggesting that algal propagation and cell cycle are dependent on the host's cell cycle. During the host's stationary growth, unicellular algal vegetatives with low chlorophyll content were dominant. In contrast, complexes of algal cells called sporangia (containing 1-4 autospores) were present in the logarithmically growing hosts, indicating that algal cell division leading to the formation of sporangia with multiple autospores is active in the dividing paramecia.
机译：草履虫草（绿色草履虫）具有内共生生长的小球藻样绿藻。从绿色MB-1菌株和除草剂百草枯制备了法氏囊球菌（MBw-1）的共生细胞系。使用共生藻类的SA-2克隆重新感染MBw-1细胞，从而获得了重新绿化的细胞系（MBr-1）。使用经过修复的草履虫来研究寄主的生长状况对共生藻类生命周期的影响。首先，通过计数在宿主细胞分裂期间和之后以及在静止期中单个宿主细胞中的藻类细胞，研究了藻类繁殖的着色与宿主细胞分裂之间的关系。其次，通过流式细胞术和荧光显微镜监测共生藻类中内源性叶绿素水平，DNA含量和细胞大小的变化。藻类的数量在宿主细胞分裂之前或期间显示为翻倍，并且两个子细胞中的藻类种群保持恒定水平，直到宿主细胞周期重新进入胞质分裂，这表明藻类繁殖和细胞周期取决于宿主的细胞周期。在宿主的静态生长过程中，叶绿素含量低的单细胞藻类营养素占主导地位。相反，在对数生长的宿主中存在称为孢子囊的藻类细胞复合物（含有1-4个自生孢子），这表明导致分裂孢子囊的藻细胞分裂导致具有多个自生孢子的孢子形成。

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