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Nuclear ploidy is contingent on the microtubular cycle responsible for plant cytokinesis

机译:核倍性取决于负责植物胞质分裂的微管循环

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Division of the plant cell relies on the preprophase band of microtubules (PPB)-phragmoplast system. Cells of onion (Allium cepa L.) root meristems were rendered binucleate by preventing the consolidation of cell plate formation in telophase with 5 mM caffeine. These binucleates developed either a single PPB around one of their two nuclei or two PPBs, one per nucleus, in the prophase of the ensuing mitosis. Prophase cells developing one single PPB were shorter in length (42.3+/-4.1 mum) than those developing 2 PPBs (49.8+/-4.1 mum), and interphase duration was inversely related to cell length. Cells whose length was less than or equal to 42 mum, i.e., which had not even reached the mean size of the small binucleates in prophase, were followed throughout mitosis. In metaphase, they always assembled two mitotic spindles (one per nucleus). However, the cells that had assembled a single PPB also developed a single phragmoplast in telophase, leading to polyploidization. As these meristematic cells were not wide enough to accommodate the midzones of both mitotic spindles in any single plane transversal to the cell elongation axis, the spindles tilted until their midzones formed a continuum where the single common phragmoplast assembled. Its position was thereby uncoupled from that of the preceding PPB. Subsequently, the chromosomes from two different half-spindles were included, by a common nuclear envelope, in a single tetraploid nucleus. Finally, the cytokinetic plate segregated the two tetraploid nuclei formed at each side of the phragmoplast into two independent sister cells.
机译:植物细胞的分裂依赖于微管(PPB)-原生质体系统的前期带。通过用5 mM咖啡因阻止在末期细胞板形成的合并,使洋葱(Allium cepa L.)根分生组织的细胞变成双核。在随后的有丝分裂的前期,这些双核或在其两个核之一附近形成单个PPB,或在每个核中形成两个PPB,每个核一个。发育一个单一PPB的前期细胞的长度(42.3 +/- 4.1微米)比发育两个PPB的前期细胞(49.8 +/- 4.1微米)短,并且相间持续时间与细胞长度成反比。在整个有丝分裂期间,追踪长度小于或等于42μm的细胞,即,其尚未达到小双核的平均大小的细胞。在中期,他们总是组装两个有丝分裂纺锤体(每个核一个)。但是,组装了单个PPB的细胞在末期也形成了单个原生质,导致多倍体化。由于这些分生细胞的宽度不足以在横穿细胞伸长轴的任何单个平面上容纳两个有丝分裂纺锤体的中间区域,因此纺锤体会倾斜直到其中间区域形成一个连续的连续体,在该连续体中单个常见的原生质膜会组装在一起。因此,它的位置与先前的PPB的位置不相关。随后,来自两个不同半轴的染色体被一个共同的核包膜包含在一个四倍体核中。最后,细胞动力学板将在原生质膜两侧形成的两个四倍体细胞核分离为两个独立的姐妹细胞。

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