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Fertilization in maize indeterminate gametophyte1 mutant

机译:玉米不确定配子体1突变体的施肥

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Mature embryo sacs of the maize mutant indeterminate gameto-phyte1 displayed different cellular patterns compared to those of the wild type. About 40% of the igl embryo sacs contained three or more synergids and two or more egg cells at the micropylar end. During fertilization in embryo sacs with two synergids. both of them frequently degenerated and were penetrated by two pollen tubes. 75% of the embryo sacs containing three or more synergid cells were penetrated by two or more pollen tubes, although most of them had only one degenerated synergid. Multiple fusions between the sperm cells and eggs frequently occurred in the same embryo sac, which subsequently generated multiple embryos. There were two or more central cells in about 33% of igl embryo sacs. The largest central cell was usually adjacent to the egg apparatus and contained two unfused polar nuclei, while those extra central cells located at the chalazal end usually had a single nucleus. Fertilization occurred only between the male gamete and the largest binucleate central cell. The extra central cells eventually degenerated after fertilization.
机译:玉米突变体的不确定胚囊的成熟胚囊与野生型相比显示出不同的细胞模式。大约40%的igl胚囊在毛孔末端包含三个或更多的协同体和两个或更多的卵细胞。受精过程中有两个协同体的胚囊。它们都经常变质并被两个花粉管穿透。含有三个或三个以上协同细胞的胚囊中有75%被两个或多个花粉管穿透,尽管其中大多数只有一个退化的协同细胞。精子细胞与卵之间的多次融合经常发生在同一胚囊中,随后产生了多个胚。在约33%的igl胚囊中有两个或多个中央细胞。最大的中央细胞通常与卵子装置相邻,并包含两个未融合的极核,而位于查拉兹尔末端的那些额外的中央细胞通常具有单个核。受精仅发生在雄配子和最大的双核中央细胞之间。受精后额外的中央细胞最终退化。

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