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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Effects of simulated microgravity on male gametophyte of Prunus, Pyrus, and Brassica species
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Effects of simulated microgravity on male gametophyte of Prunus, Pyrus, and Brassica species

机译：模拟微重力对李，梨和芸苔属植物雄配子体的影响

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摘要

In this study we evaluated the effect of simulated microgravity on pollen germination of both herbaceous and woody species in order to investigate the possibility of applying gametophytic selection for plant growth in the space environment. The behaviour of gametophytes exposed to the stress of clinostat rotation could be used to screen the degree of tolerance of the sporophyte to simulated microgravity. The use of male gametophyte selection overcomes the problems generally encountered by sporophytic selection in space especially for woody plants: the large size of plants and their long juvenile phase. In this experiment, pollen collected from just bloomed flowers of Prunus persica, P. avium, P. domestica, Pyrus communis, and Brassica rapa was subjected to tests assessing its viability by techniques such as fluorochromatic reaction. Once pollen viability was ascertained by fluorescence microscopy, pollen was placed on the growth medium in petri dishes both at 1 g and on the clinostat. After incubating for 1 day at room temperature, pollen was observed under a light microscope in order to detect parameters such as the percentage of germination and the growth direction. Then histochemical analyses were performed in order to verify the presence and distribution of nuclei, cytoplasm, and storage substances. Moreover, the presence, size, and morphology of callose plugs were observed. Results showed that the response of gametophytes to simulated microgravity is dependent on the species, some showing altered metabolism, others being unaffected.
机译：在这项研究中，我们评估了模拟微重力对草本和木本植物花粉萌发的影响，以研究将配子植物选择应用于空间环境中植物生长的可能性。暴露于斜管旋转应力下的配子体的行为可用于筛选孢子体对模拟微重力的耐受程度。雄配子体选择的使用克服了空间孢子选择所普遍遇到的问题，特别是对于木本植物：植物大且它们的幼年期长。在该实验中，对从李，李子，家蝇，野梨和芸苔的刚开花的花中采集的花粉进行了测试，通过荧光反应等技术对其活力进行了评估。一旦通过荧光显微镜确定了花粉的生存力，就将花粉以1g和在clinostat上置于培养皿中的生长培养基上。在室温下孵育1天后，在光学显微镜下观察花粉以检测诸如发芽百分比和生长方向的参数。然后进行组织化学分析，以验证核，细胞质和存储物质的存在和分布。此外，观察到call形塞的存在，大小和形态。结果表明配子体对模拟微重力的反应取决于物种，有些表现出新陈代谢的改变，另一些则不受影响。

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