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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Mucor rouxii ultrastructure: cyclic AMP and actin cytoskeleton
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Mucor rouxii ultrastructure: cyclic AMP and actin cytoskeleton

机译：鲁氏双球菌超微结构：环状AMP和肌动蛋白细胞骨架

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摘要

A comparative analysis of the effect of two compounds, dibutyryl-cyclic-AMP (dbcAMP) and latrunculin B, on the morphology and ultrastructure of the dimorphic fungus Mucor rouxii under aerobic growth conditions is presented. dbcAMP acts through the sustained activation of protein kinase A, and latrunculin B through the disruption of the actin cytoskeleton. Upon addition of these compounds to the growth medium at any stage of the germination process, cells lost polarised growth and switched to isodiametric growth. The effect was reversible. The morphologies, visualised by light microscopy or scanning electron microscopy (SEM), were alike. A switch from a rough to a smooth surface was observed by SEM when cells were repolarised by removal of the added compound. Ultrastructural changes under both conditions, as observed by transmission electron microscopy, were similar, the main feature being the enlargement of the cell wall, with irregular depositions, and detachment from the cell membrane. dbcAMP-treated cells showed a decrease in the number of glycogen granules compared with control and latrunculin B-treated cells. F-actin staining with fluorescein isothiocyanate-phalloidin showed that both dbcAMP- and latrunculin B-treated cells displayed a much lower fluorescence than control cells, with only a few pale plaques. The results suggest that the sustained activation of protein kinase A, which impairs polarised growth, might exert its effect through a modification of actin cytoskeleton organisation, very probably also involving an integrinlike pathway, as judged by the cell wall detachment and loss of cell adhesiveness of the dbcAMP-treated isodiametric cells.
机译：提出了两种化合物，二丁酰环-AMP（dbcAMP）和latrunculin B对有氧生长条件下双态真菌Mucor rouxii形态和超微结构影响的比较分析。 dbcAMP通过蛋白激酶A的持续活化来发挥作用，而通过肌动蛋白的细胞骨架的破坏来维持拉特朗菌素B的作用。在萌发过程的任何阶段将这些化合物添加到生长培养基中后，细胞就会失去极化的生长并转换为等径生长。效果是可逆的。通过光学显微镜或扫描电子显微镜（SEM）观察的形态相似。当通过去除添加的化合物使细胞重新极化时，通过SEM观察到从粗糙表面到光滑表面的转变。通过透射电子显微镜观察，在两种条件下的超微结构变化是相似的，主要特征是细胞壁的扩大，不规则的沉积以及与细胞膜的分离。 dbcAMP处理的细胞显示糖原颗粒的数量与对照组和经latrunculin B处理的细胞相比有所减少。用荧光素异硫氰酸酯-鬼笔环肽进行的F-肌动蛋白染色显示，经dbcAMP处理和经latrunculin B处理的细胞均显示出比对照细胞低得多的荧光，只有很少的淡斑。结果表明，蛋白激酶A的持续活化会损害极化生长，可能通过肌动蛋白细胞骨架组织的修饰发挥其作用，这很可能还涉及整合素样途径，这可以通过细胞壁的脱落和细胞粘附性的丧失来判断。 dbcAMP处理的等径细胞。

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