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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Microautoradiographic localisation of [H-3]sucrose and [H-3]mannitol in Robinia pseudoacacia pulvinar tissues during phytochrome-mediated nyctinastic closure
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Microautoradiographic localisation of [H-3]sucrose and [H-3]mannitol in Robinia pseudoacacia pulvinar tissues during phytochrome-mediated nyctinastic closure

机译：在植物色素介导的夜光子闭合过程中刺槐刺槐组织中[H-3]蔗糖和[H-3]甘露醇的微放射自显影定位

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摘要

We have analysed the incorporation of [H-3]sucrose and [H-3]mannitol in pulvinar motor cells of Robinia pseudoacacia L. during phytochrome-mediated nyctinastic closure. Pairs of leaflets, excised 2 h after the beginning of the photoperiod, were fed with 50 mM [H-3]sucrose or [H-3]mannitol, irradiated with red (15 min) or far-red (5 min) light and placed in the dark for 2-3 h. Label uptake was measured in whole pulvini by liquid scintillation counting. The distribution of labelling in pulvinar sections was assessed by both light and electron microautoradiography. [H-3]Sucrose uptake was twice that of [H-3]mannitol incorporation in both red- and far-red-irradiated pulvini. In the autoradiographs, [H-3]sucrose and [(3H)]mannitol labelling was localised in the area from the vascular bundle to the epidermis, mainly in vacuoles, cytoplasm, and cell walls. Extensor and flexor protoplasts displayed a different distribution of [H-3]sucrose after red and far-red irradiation. Far-red light drastically reduced the [H-3]sucrose incorporation in extensor protoplasts and caused a slight increase in internal flexor protoplasts. After red light treatment, no differences in [H-3]sucrose labelling were found between extensor and flexor protoplasts. Our results indicate a phytochrome control of sucrose distribution in cortical motor cells and seem to rule out the possibility of sucrose acting as an osmoticum.
机译：我们已经分析了[H-3]蔗糖和[H-3]甘露糖醇在植物色素介导的夜光子闭合过程中在刺槐刺槐运动细胞中的掺入。在光周期开始后2小时将一对成对的小叶喂以50 mM [H-3]蔗糖或[H-3]甘露醇，用红色（15分钟）或远红色（5分钟）照射，在黑暗中放置2-3小时。通过液体闪烁计数测量整个pulvini中的标签摄取。通过光和电子显微放射自显影来评估在肺泡切片中标记的分布。 [H-3]蔗糖的摄取量是红和远红pulvini中[H-3]甘露醇掺入量的两倍。在放射自显影照片中，[H-3]蔗糖和[（3H）]甘露醇标记位于从血管束到表皮的区域，主要位于液泡，细胞质和细胞壁中。在红色和远红外线照射后，伸肌和屈肌原生质体显示出不同的[H-3]蔗糖分布。远红外光大大减少了伸肌原生质体中[H-3]蔗糖的掺入，并导致内部屈肌原生质体略有增加。经过红光处理后，伸肌和屈肌原生质体在[H-3]蔗糖标记上没有发现差异。我们的结果表明植物色素可控制蔗糖在皮质运动细胞中的分布，并似乎排除了蔗糖作为渗透剂的可能性。

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