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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Inositol 1,4,5-trisphosphate and Ran expression during simulated and real microgravity
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Inositol 1,4,5-trisphosphate and Ran expression during simulated and real microgravity

机译：模拟和真实微重力下的肌醇1,4,5-三磷酸和Ran表达

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摘要

In order to gain further insight into the signal transduction pathway concerning gravitropism, we studied the expression profiles of mRNA in etiolated Sunflower (Helianthus annims L.) seedlings. Differential-display reverse transcriptase PCR product assayed by capillary electrophoresis revealed the small GTPase Ran, regulating nuclear import and export of proteins. Parallel analysis of inositol 1,4,5-trisphosphate (Ins(1,4,5)P,) release by a highly advanced system of metal-dye detection combined with high-performance liquid chromatography provided evidence that the second messenger Ins(1,4,5)p3 is modulated by changes of the gravity vector. Investigations by fast clinorotation and sounding rockets established a positive correlation between the Ins(1,4,5)P-3 level and the expression rate of Ran mRNA during simulated and real microgravity. Since an asymmetric distribution of auxin during graviresponse is suggested to induce differential cell elongation, additional information on the perception and transduction pathways was achieved by auxin stimulation experiments. While we were able to demonstrate an auxin-dependent production of Ins(1,4,5)p3, the expression of Ran mRNA was not affected by auxin. Finally. besides the phosphoinositide system as one element of the signal transduction chain linking graviperception to graviresponse, a Ran-mediated interaction model of extracellular microgravity signal perception and intercellular transduction pathway is proposed.
机译：为了进一步了解有关重力的信号转导途径，我们研究了黄化向日葵（Helianthus annims L.）幼苗中mRNA的表达谱。通过毛细管电泳分析的差异展示逆转录酶PCR产物显示出小的GTPase Ran，调节蛋白质的核输入和输出。通过高度先进的金属染料检测系统与高效液相色谱系统对肌醇1,4,5-三磷酸（Ins（1,4,5）P，）释放进行平行分析，提供了第二种信使Ins（1 ，4,5）p3通过重力矢量的变化进行调制。通过快速旋转和探测火箭的研究，在模拟和真实微重力作用下，Ins（1,4,5）P-3水平与Ran mRNA的表达率之间存在正相关。由于建议在重力反应过程中生长素的不对称分布会诱导细胞的差异伸长，因此通过生长素刺激实验可获得有关知觉和转导途径的其他信息。虽然我们能够证明Ins（1,4,5）p3的生长素依赖性生产，但Ran mRNA的表达不受生长素的影响。最后。除了磷酸肌醇系统作为重力感应与重力反应连接的信号转导链的一个要素外，还提出了一种Ran介导的细胞外微重力信号感知与细胞间转导途径的相互作用模型。

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