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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Cellular change and callose accumulation in zygotic embryos of Eleutherococcus senticosus caused by plasmolyzing pretreatment result in high frequency of single-cell-derived somatic embryogenesis
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Cellular change and callose accumulation in zygotic embryos of Eleutherococcus senticosus caused by plasmolyzing pretreatment result in high frequency of single-cell-derived somatic embryogenesis

机译：纤溶酶预处理导致的刺肠肠球菌合子胚中的细胞变化和call质积累导致单细胞来源的体细胞胚发生的高频率

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摘要

Eleutherococcus senticosus zygotic embryos were pretreated with 1.0 M mannitol or sucrose for 3-24 h. This pretreatment resulted in a high frequency of somatic-embryo formation on hormone-free medium. All the somatic embryos developed directly and independently from single epidermal cells on the surface of zygotic embryos after plasmolyzing pretreatment. Scanning electron microscopic observation revealed that the epidermal cells of hypocotyls rapidly became irregular and showed a random orientation before somatic-embryo development commenced. At the same time, the epidermal cells in the untreated control remained regular. Callose concentration determined by fluorometric analysis increased sharply in E. senticosus zygotic embryos after plasmolyzing pretreatment but remained lose in the untreated control. Aniline blue fluorescent staining of callose showed that the plasmolyzing pretreatment of zygotic embryos resulted in heavy accumulation of callose between the plasma membrane and cell walls. On the basis of these results, we suggest that plasmolyzing pretreatment of zygotic embryos induces the accumulation of callose, and the interruption of cell-to-cell communication imposed by this might stimulate the reprogramming of epidermal cells into embryogenically competent cells and finally induce somatic-embryo development from single cells.
机译：用1.0 M甘露醇或蔗糖预处理Eeletherococcus senticosus合子胚3-24小时。这种预处理导致无激素培养基上的体细胞胚形成频率很高。在进行了溶质分解的预处理后，所有体细胞胚均直接和独立地从合子胚表面的单个表皮细胞发育而来。扫描电镜观察发现，胚轴的表皮细胞迅速变得不规则，并在体胚发育开始之前表现出随机取向。同时，未经处理的对照中的表皮细胞保持规则。荧光分析法测定的愈浓度在纤溶酶预处理后在E. senticosus合子胚中急剧增加，但在未处理的对照中仍然丢失。 ose质的苯胺蓝荧光染色表明，对合子胚进行溶酶预处理可导致质膜和细胞壁之间大量积聚call质。根据这些结果，我们建议对合子胚进行胞浆裂解预处理会诱导of质的积累，由此造成的细胞间通讯中断可能会刺激表皮细胞重编程为胚性感受态细胞，并最终诱导体细胞分化。单细胞的胚胎发育。

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