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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Androgenic response to preculture stress in microspore cultures of barley
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Androgenic response to preculture stress in microspore cultures of barley

机译：大麦小孢子培养中雄激素对培养前胁迫的响应

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摘要

Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic microspore development could be induced from treated and nontreated microspores, abiotic stress was essential for embryo formation and plant regeneration. The type of stress treatment applied affected the numbers and the ratios of albino and green plants regenerated, as well as their fertility. The highest number of green plants was obtained after the treatment of anthers in 0.3 M mannitol at 32 degrees C for 24 h before microspore culture.
机译：各种压力，例如饥饿和冷或热休克已被确定为诱导小孢子胚胎发生的诱因。这项研究试图量化不同预处理条件对麦芽大麦（cv。Scarlett）成功进行小孢子培养的影响。虽然可以从处理和未处理的小孢子中诱导孢子状小孢子的发育，但非生物胁迫对于胚胎形成和植物再生至关重要。施加的胁迫处理类型影响了再生的白化植物和绿色植物的数量和比例，以及它们的繁殖力。在小孢子培养之前，将花药在32 M的0.3 M甘露醇中处理过24小时后，可获得最高数量的绿色植物。

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