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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Detection and release of allergenic proteins in Parietaria judaica pollen grains
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Detection and release of allergenic proteins in Parietaria judaica pollen grains

机译：检测和释放Parietaria judaica花粉粒中的致敏蛋白

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摘要

Rapid diffusion of allergenic proteins in isotonic media has been demonstrated for different pollen grains. Upon contact with stigmatic secretion or with the mucosa of sensitive individuals, pollen grains absorb water and release soluble low-molecular-weight proteins, these proteins enter in the secretory pathway in order to arrive at the cell surface. In this study we located allergenic proteins in mature and hydrated-activated pollen grains of Parietaria judaica L. (Urticaceae) and studied the diffusion of these proteins during the first 20 min of the hydration and activation processes. A combination of transmission electron microscopy and immunocytochemical methods was used to locate these proteins in mature pollen and in pollen grains after different periods of hydration and activation processes. Activated proteins reacting with antibodies in human serum from allergic patients were found in the cytoplasm, wall, and exudates from the pollen grains. The allergenic component of these pollen grains changes according to the pollen state; the presence of these proteins in the exine, the cytoplasm, and especially in the intine and in the material exuded from the pollen grains, is significant in the hydrated-activated studied times, whereas this presence is not significant in mature pollen grains. The rapid activation and release of allergenic proteins of P. judaica pollen appears to be the main cause of the allergenic activity of these pollen grains.
机译：对于不同的花粉粒，已证明变应原蛋白在等渗介质中的快速扩散。一旦接触到光头分泌物或敏感个体的粘膜，花粉颗粒就会吸收水分并释放出可溶性的低分子量蛋白质，这些蛋白质会进入分泌途径，从而到达细胞表面。在这项研究中，我们将变应原蛋白定位在Parietaria judaica L.（荨麻科）的成熟和水合激活花粉粒中，并研究了这些蛋白在水合和激活过程的前20分钟内的扩散。结合透射电子显微镜和免疫细胞化学方法，在水化和活化过程不同的时间后，将这些蛋白质定位在成熟的花粉中和花粉粒中。在花粉粒的细胞质，壁和分泌物中发现了与过敏患者人血清中的抗体反应的活化蛋白。这些花粉粒的致敏成分根据花粉状态而变化;在水合活化的研究时间内，这些蛋白质在外在，细胞质中的存在，特别是在花粉粒中的intine和材料中的存在是很重要的，而在成熟的花粉粒中这种存在并不显着。美洲山茱poll花粉的致敏蛋白的快速活化和释放似乎是这些花粉粒的致敏活性的主要原因。

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