...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Diurnal differences in the supply of glucomannans and xylans to innermost surface of cell walls at various developmental stages from cambium to mature xylem in Cryptomeria japonica
【24h】

Diurnal differences in the supply of glucomannans and xylans to innermost surface of cell walls at various developmental stages from cambium to mature xylem in Cryptomeria japonica

机译：日本柳杉从形成层到成熟木质部在不同发育阶段向细胞壁最内表面供应葡甘露聚糖和木聚糖的昼夜差异

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated the diurnal differences in the innermost surface of tracheid cell walls at various developmental stages from cambium to mature xylem. Cryptomeria japonica saplings were cultivated in a growth chamber with a light cycle set at 14 h of light and 10 h of darkness. Samples were collected from the saplings during both the light and dark periods. The innermost surface of cell walls was immunogold-labeled with anti-glucomannan or anti-xylan antiserum and was observed by field emission scanning electron microscopy. Diurnal differences in the aspect of the innermost surface of cell walls were seen only in S-2-layer-forming tracheids; cellulose microfibrils were clearly evident during the light period, and amorphous material containing glucomannans and xylans was prevalent during the dark period. Cellulose microfibrils were present at the primary-wall formation and S-1-layer-forming stages, and many warts were observed in the mature tracheids, regardless of the time of sampling. The densities of labeled glucomannans on the innermost surface of cell walls in S-1- and S-2-forming tracheids and of labeled xylans in S-2-forming tracheids during the dark period were significantly higher than those during the light period. These results suggest a diurnal periodicity in the supply of cell wall matrix containing hemicellulose to the innermost surface of developing secondary walls.
机译：我们调查了从形成层到成熟木质部的各个发育阶段的气管细胞壁最内表面的昼夜差异。在生长室中培养日本柳杉幼树，光照周期设定为光照14小时和黑暗10小时。在明亮和黑暗时期都从幼树中收集样品。细胞壁的最内表面用抗葡甘露聚糖或抗木聚糖抗血清免疫金标记，并通过场发射扫描电子显微镜观察。细胞壁最内表面方面的昼夜差异仅在形成S-2层的管胞中可见;而在细胞壁的最内表面上则没有。在光亮时期，纤维素微纤维明显可见，在黑暗时期，含有葡甘露聚糖和木聚糖的无定形物质盛行。纤维素微纤维存在于初生壁形成和S-1层形成阶段，并且在成熟的气管中观察到许多疣，而与采样时间无关。在黑暗时期，在S-1和S-2形成的管胞中细胞壁最内表面的标记葡甘露聚糖的密度和在S-2形成的管胞中的标记木聚糖的密度显着高于明亮时期。这些结果表明，含有半纤维素的细胞壁基质向发育中的次生壁的最内表面的供应具有昼夜周期性。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号