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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >EFFECTS OF LEAD ON THE NUCLEAR REPETITIVE DNA OF THE MOSS FUNARIA HYGROMETRICA (BRYOPHYTA)
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EFFECTS OF LEAD ON THE NUCLEAR REPETITIVE DNA OF THE MOSS FUNARIA HYGROMETRICA (BRYOPHYTA)

机译：铅对苔藓真菌（苔藓植物）核重复DNA的影响

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摘要

funaria hygrometrica gametophytes were grown in in vitro controlled systems in the presence and absence of lead. Their nuclear genomes were then analyzed directly in situ with A+T and G+C specific fluorochromes, image analysis and statistical data elaboration by specific software, in order to characterize the different fractions of repetitive DNA. The results reveal qualitative and quantitative differences between the nuclear genomes of lead-stressed and unstressed individuals. These differences seem to consist of a significant increase in G+C rich repetitive DNA sequences in nuclei of the stressed individuals. These sequences form well defined agglomerates, generally situated adjacent to the nucleolar region, which increase in both size and number in the presence of lead. Some hypotheses are discussed. [References: 25]
机译：在有铅和无铅的情况下，在体外控制系统中生长了潮菌真菌。然后，使用A + T和G + C特定的荧光染料直接对它们的核基因组进行原位分析，并通过特定软件对图像分析和统计数据进行详细分析，以表征重复DNA的不同部分。结果揭示了铅胁迫和无胁迫个体的核基因组之间的定性和定量差异。这些差异似乎由应激个体细胞核中富含G + C的重复DNA序列的显着增加组成。这些序列形成定义明确的附聚物，通常位于核仁区域附近，在存在铅的情况下其大小和数量均增加。讨论了一些假设。 [参考：25]

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