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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >THE MONOCLONAL ANTIBODY JIM5 INDICATES PATTERNS OF PECTIN DEPOSITION IN RELATION TO PIT FIELDS AT THE PLASMA-MEMBRANE-FACE OF TOMATO PERICARP CELL WALLS
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THE MONOCLONAL ANTIBODY JIM5 INDICATES PATTERNS OF PECTIN DEPOSITION IN RELATION TO PIT FIELDS AT THE PLASMA-MEMBRANE-FACE OF TOMATO PERICARP CELL WALLS

机译：单克隆抗体JIM5指示果胶周壁细胞壁质膜坑表面果胶沉积的形态

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摘要

The use of the anti-pectin monoclonal antibody JIM5 in conjunction with immunofluorescence microscopy and also confocal microscopy has indicated that the JIM5 epitope is associated with structural features of the plasma-membrane-face of the cell wall of tomato pericarp cells. JIM5 recognized the primary pit fields of the cell walls, as identified by co-staining with callose-reactive aniline blue. In addition, abundant linear arrays of the pectin epitope were observed to radiate out from the primary pit fields in parallel, as well as random, arrangements. These observations have implications for our understanding of the organization of the polymer networks that comprise the primary cell wall. [References: 16]
机译：抗果胶单克隆抗体JIM5结合免疫荧光显微镜和共聚焦显微镜的使用表明，JIM5表位与番茄果皮细胞的细胞壁的质膜表面结构特征有关。 JIM5识别了细胞壁的主要凹坑区域，这是通过与call质反应性苯胺蓝共染色确定的。另外，观察到大量的果胶表位线性阵列以平行和随机排列的方式从初级基坑场辐射出来。这些观察结果对我们对构成原代细胞壁的聚合物网络的组织结构的理解具有影响。 [参考：16]

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