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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >ULTRASTRUCTURE OF CHYTRIDIOMYCETE AND OOMYCETE ZOOSPORES USING SPRAY-FREEZE FIXATION
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ULTRASTRUCTURE OF CHYTRIDIOMYCETE AND OOMYCETE ZOOSPORES USING SPRAY-FREEZE FIXATION

机译：喷雾-冷冻固定法检出藻类和卵菌纲的超微结构

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摘要

The ultrastructure of zoospores of several zoosporic fungi was examined using a modified cryofixation technique. An atomizer was used to spray a zoospore suspension into the cold propane reservoir of a conventional plunge freeze-substitution apparatus. Spray-freeze fixation and freeze-substitution of zoospores por porvided better fixation of vacuolar structures, membranes and the extracellular coat than that obtained with chemical fixation. The overall shape of cryofixed spores was closer to that seen in living zoospores. Two types of vacuoles were seen in cryofixed zoospores of Monoblepharella and Chytridium. One type of vacuole contained electron-opaque material within the lumen while the other type had no visible internal material in the lumen and appeared to be part of the water expulsion vacuole complex. Coated pits and coated vesicles were observed associated with both the water expulsion vacuoles and the plasma membrane in Monoblepharella and Phytophthora, suggesting that endocytosis of the plasma membrane and expulsion vacuoles is part of membrane recycling during osmoregulatory events. An extracellular coat was seen on the outer surface of cryofixed zoospores of Monoblepharella sp., Chytridium confervae and Phytophthora palmivora without the use of carbohydrate-specific stains. The spray-freeze method gave good and reproducible fixation of the wall-less spores in quantities greater than those obtained in previously described zoospore cryofixation studies. The technique is potentially useful for cell suspensions in that freeze damage from excess water is limited. [References: 42]
机译：使用改良的冷冻固定技术检查了几种游动孢子真菌游动孢子的超微结构。使用雾化器将游动孢子悬浮液喷雾到常规的急冻冷冻替代设备的冷丙烷储器中。游动孢子的喷雾冷冻固定和冷冻替代使液泡结构，膜和细胞外被膜的固定比化学固定得到的固定更好。冷冻固定孢子的整体形状更接近于活的游动孢子。在低温固定的单孢菌和梭菌的游动孢子中观察到两种类型的液泡。一种类型的液泡在管腔内包含电子不透明材料，而另一种类型的液泡在管腔内没有可见的内部材料，并且似乎是排水液泡复合物的一部分。观察到包膜的凹坑和包膜的囊泡与单卵小球藻和疫霉中的水分排出液泡和质膜都相关，表明质膜内吞和排出液泡是渗透压调节过程中膜再循环的一部分。在不使用碳水化合物特异的染色剂的情况下，在Monoblepharella sp。，商球梭菌和Phytophthora palmivora的冷冻固定游动孢子的外表面上看到了细胞外被膜。喷雾冷冻法可对无壁孢子进行良好且可重现的固定，其数量要大于先前描述的游动孢子冷冻固定研究中获得的数量。该技术对于细胞悬浮液可能是有用的，因为来自过量水的冷冻破坏是有限的。 [参考：42]

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