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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The cytoplasmic domain of the cellulose-synthesizing complex in vascular plants
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The cytoplasmic domain of the cellulose-synthesizing complex in vascular plants

机译：维管植物中纤维素合成复合物的胞质结构域

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摘要

The cytoplasmic domain of the rosette terminal complex has been imaged in situ in patches of plasma membrane isolated from tobacco BY-2 protoplasts. By partially extracting the plasma membrane lipids, cellulose microfibrils were observed through the plasma membrane. Rosette terminal complexes were identified on the basis of their association with the ends of these cellulose microfibrils. The cytoplasmic domain of the rosette terminal complex has been shown to be hexagonal in shape and has been measured to be 45-50 nm in diameter and 30-35 nm tall. These findings demonstrate that the terminal complex does indeed have a substantial cytoplasmic component, and that the hexagonal array observed in the lipid bilayer by freeze fracture is actually only a small part of the overall complex. These findings will allow better modeling of the terminal complex and may facilitate predictions of how many proteins are associated with the rosette terminal complex in vivo.
机译：玫瑰花结末端复合物的胞质结构域已在从烟草BY-2原生质体中分离的质膜上进行了原位成像。通过部分提取质膜脂质，通过质膜观察到纤维素微纤维。玫瑰花结末端复合物是基于它们与这些纤维素微纤维末端的结合而鉴定的。玫瑰花结末端复合物的胞质结构域已显示为六边形，并已测量为直径45-50 nm和高30-35 nm。这些发现表明，末端复合物确实具有实质性的细胞质成分，并且通过冷冻断裂在脂质双层中观察到的六边形阵列实际上仅是整个复合物的一小部分。这些发现将允许对末端复合物进行更好的建模，并且可以促进体内与莲座状末端复合物相关联的蛋白质的预测。

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