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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Recovery of microtubules on the blepharoplast of Ceratopteris spermatogenous cells after oryzalin treatment
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Recovery of microtubules on the blepharoplast of Ceratopteris spermatogenous cells after oryzalin treatment

机译：稻草素处理后尾翅目生精细胞原生质细胞微管的回收

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摘要

Most land plants have ill-defined microtubule-organizing centers (MTOCs), consisting of sites on the nuclear envelope or even along microtubules (MTs). In contrast, the spermatogenous cells of the pteridophyte Ceratopteris richardii have a well-defined MTOC, the blepharoplast, which organizes MTs through the last two division cycles. This allows a rare opportunity to study the organization and workings of a structurally well-defined plant MTOC. In this study, antheridial plants were treated with levels of oryzalin that cause complete MT loss from the cells containing blepharoplasts. The oryzalin was then washed out and plants were allowed to recover for varying amounts of time. If the spermatogenous cells were fixed prior to washing out, the blepharoplasts had an unusual appearance. In the matrix (pericentriolar) material where MT ends are normally found, clear areas of about the diameter of MTs were seen embedded in a much deeper matrix, made more obvious in stereo pairs. Occasionally, the matrix material was highly distended, although the basal body template cylinder morphology appeared to be unaltered. The blepharoplasts often occurred as clusters of 2 or 4, indicating that blepharoplast reproduction is not affected by the lack of MTs, but that their movement to the poles is. Gamma (gamma) tubulin antibodies labeled the edge of the blepharoplast in areas where the pits are located, indicating that these might be sites for MT nucleation. After wash out, the new MTs always re-appeared on the blepharoplast and the recovery occurred within an hour of washout. MT lengths increased with increasing washout time and were indistinguishable from untreated blepharoplasts after 24 h of recovery. After washout, arrays formed in new sperm cells such as the spline and basal bodies were often malformed or present in multiple copies, as were the blepharoplasts in these cells prior to wash out. These data indicate that the blepharoplast serves as the site of MT nucleation and organization even after complete MT de-polymerization.
机译：大多数陆地植物都有定义不明确的微管组织中心（MTOC），由核膜上甚至微管（MTs）上的位点组成。相比之下，蕨类蕨类植物Ceratopteris richardii的生精细胞具有定义明确的MTOC，即原生质细胞，它在最后两个分裂周期内组织了MT。这为研究结构明确的工厂MTOC的组织和运作提供了难得的机会。在这项研究中，用高水平的稻草素处理过的花药，使它们从含有原生质塑料的细胞中完全消失。然后洗掉米草素，使植物恢复不同的时间。如果在洗出之前先将生精细胞固定下来，则原生质体的外观会异常。在通常发现MT末端的基质（周质）材料中，可以看到大约MT直径的透明区域嵌入了更深的基质中，这在立体对中更为明显。有时，基体材料高度膨胀，尽管基体模板圆柱体的形态似乎没有改变。原生质体通常以2或4的簇出现，表明原生质体的繁殖不受MT缺乏的影响，但它们向极点的运动却受到影响。伽玛（gamma）微管蛋白抗体标记了凹坑所在区域的原生质塑料边缘，表明这些可能是MT成核的位点。冲洗后，新的MT总是重新出现在原生质体上，并且在冲洗后一小时内就恢复了。 MT长度随着洗脱时间的增加而增加，并且在恢复24小时后与未处理的原生质体没有区别。冲洗后，在新的精子细胞中形成的阵列（如样条和基体）通常畸形或以多份形式存在，冲洗前这些细胞中的原生质体也是如此。这些数据表明，即使在完全MT解聚后，珠光体仍是MT成核和组织的位点。

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