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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Cytological investigation of Haplopappus gracilis (Nutt.) Gray: 5-methylcytosine-rich regions, fluorochrome banding and chromatin sensitivity to DNase I digestion
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Cytological investigation of Haplopappus gracilis (Nutt.) Gray: 5-methylcytosine-rich regions, fluorochrome banding and chromatin sensitivity to DNase I digestion

机译：轻链河豚的细胞学研究（灰色）：富含5-甲基胞嘧啶的区域，荧光染料条带和染色质对DNase I消化的敏感性

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摘要

Haplopappus gracilis (Nutt.) Gray, one of the five known higher plants with a chromosome number of 2n = 4, was studied from a cytological point of view. The chromosome complement of this species was characterized by means of automated karyotype analysis. Moreover, the DNA methylation pattern and fluorochrome banding were determined and compared with cytological data present in the literature. DNA methylation distribution along metaphase chromosomes involved all chromosome territories evidenced by C-banding. Other methylated bands correlated positively with aceto-orcein-positive heterochromatic portions and/or with late replicating bands and/or fluorochrome bands. Some methylated bands showed differences between homologous chromosomes. These bands belonged partly to certain heterochromatic domains and partly to intercalary sites not defined by other standard banding techniques. Differences between the homologues were also indicated by our DNA content data obtained after DNase I digestion.
机译：从细胞学角度研究了Haplopappus gracilis（Nutt。）Gray，它是五种已知的高等植物之一，其染色体数为2n = 4。该物种的染色体补体通过自动核型分析来表征。此外，确定了DNA甲基化模式和荧光色素带，并将其与文献中存在的细胞学数据进行了比较。沿中期染色体的DNA甲基化分布涉及C谱带证明的所有染色体区域。其他甲基化的条带与乙酰大黄素阳性的异色部分和/或与后期复制条带和/或荧光染料条带正相关。一些甲基化带显示同源染色体之间的差异。这些条带部分属于某些异色域，部分属于其他标准谱带技术未定义的cal间位点。 DNase I消化后获得的DNA含量数据也表明了同源物之间的差异。

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