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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Metallothionein-like gene from Cicer microphyllum is regulated by multiple abiotic stresses
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Metallothionein-like gene from Cicer microphyllum is regulated by multiple abiotic stresses

机译：小叶蝉的金属硫蛋白样基因受多种非生物胁迫调控

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摘要

Cicer microphyllum, a wild relative of cultivated chickpea, is a high altitude cold desert-adapted species distributed in western and trans-Himalayas. A complementary DNA (cDNA) encoding metallothionein-like protein has been identified from a cold-induced subtraction cDNA library from C. microphyllum. The sequence of the cloned metallothionein gene from C. microphyllum (GQ900702) contains 240-bp-long open reading frame and encodes predicted 79-amino acid protein of 7. 9 kDa. Sequence analysis identified the motifs characteristic of type II metallothionein and designated as CmMet-2. Southern hybridization confirms a single copy of the CmMet-2 gene in C. microphyllum genome. In situ hybridization indicated spatial transcript regulation of CmMet-2 in root and aerial parts and also confirmed through real-time PCR-based quantitative transcript analysis. The data revealed a significantly low level of transcript in the aerial parts than the roots. Quantitative analysis using real-time PCR assay revealed induction of transcript in all parts of plants in response to cold stress at 4°C. The transcript abundance was found to increase exponentially with time course from 6 to 24 h after exposure. Further, regulation of transcript accumulation in response to abscisic acid application, polyethylene glycol (100 μM)-induced osmotic stress, or ZnSO_4 (1 μM) foliar spray indicated by Northern hybridization suggests the involvement of CmMet-2 in multiple stress response.
机译：小鹰嘴豆是栽培鹰嘴豆的野生近缘种，是分布在西部和反喜马拉雅山的一种高海拔寒冷沙漠适应种。已经从冷诱导的小叶梭菌cDNA文库中鉴定了编码金属硫蛋白样蛋白的互补DNA（cDNA）。克隆自小叶梭菌的金属硫蛋白基因（GQ900702）的序列包含240 bp长的开放阅读框，编码7. 9 kDa的预测79个氨基酸蛋白。序列分析鉴定了II型金属硫蛋白特征的基序，并命名为CmMet-2。 Southern杂交证实了小叶梭菌基因组中CmMet-2基因的单拷贝。原位杂交表明CmMet-2在根和空中部分的空间转录调控，并通过基于实时PCR的定量转录分析得到证实。数据显示，气生部的转录本水平明显低于根部。使用实时PCR分析的定量分析显示，在4°C的冷胁迫下，植物所有部位均诱导了转录本。发现在暴露后6至24小时内，转录物的丰度随时间进程呈指数增长。此外，Northern杂交表明，响应于脱落酸施用，聚乙二醇（100μM）引起的渗透胁迫或ZnSO_4（1μM）叶面喷雾对转录物积累的调节表明CmMet-2参与了多种胁迫反应。

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